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Analysis of volatile organic compounds liberated and metabolised by human umbilical vein endothelial cells (HUVEC) in vitro.

Cell biochemistry and biophysics (2014-08-16)
Paweł Mochalski, Markus Theurl, Andreas Sponring, Karl Unterkofler, Rudolf Kirchmair, Anton Amann
ABSTRACT

Gas chromatography with mass spectrometric detection combined with head-space needle trap extraction as the pre-concentration technique was applied to identify and quantify volatile organic compounds released or metabolised by human umbilical vein endothelial cells. Amongst the consumed species there were eight aldehydes (2-methyl 2-propenal, 2-methyl propanal, 2-methyl butanal, 3-methyl butanal, n-hexanal, benzaldehyde, n-octanal and n-nonanal) and n-butyl acetate. Further eight compounds (ethyl acetate, ethyl propanoate, ethyl butyrate, 3-heptanone, 2-octanone, 2-nonanone, 2-methyl-5-(methylthio)-furan and toluene) were found to be emitted by the cells under study. Possible metabolic pathways leading to the uptake and release of these compounds by HUVEC are proposed and discussed. The uptake of aldehydes by endothelial cells questions the reliability of species from this chemical class as breath or blood markers of disease processes in human organism. The analysis of volatiles released or emitted by cell lines is shown to have a potential for the identification and assessment of enzymes activities and expression.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Toluene, anhydrous, 99.8%
Sigma-Aldrich
Ethyl acetate, anhydrous, 99.8%
Sigma-Aldrich
Ethyl acetate, ACS reagent, ≥99.5%
Sigma-Aldrich
Benzaldehyde, ReagentPlus®, ≥99%
Sigma-Aldrich
Ethyl acetate, suitable for HPLC, ≥99.7%
Sigma-Aldrich
Toluene, ACS reagent, ≥99.5%
Sigma-Aldrich
Ethyl acetate, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.5% (GC)
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Toluene, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.7% (GC)
Sigma-Aldrich
Benzaldehyde, purified by redistillation, ≥99.5%
Sigma-Aldrich
Toluene, suitable for HPLC, 99.9%
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Ethyl acetate, HPLC Plus, for HPLC, GC, and residue analysis, 99.9%
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Toluene, HPLC Plus, for HPLC, GC, and residue analysis, ≥99.9%
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Butyl acetate, anhydrous, ≥99%
Sigma-Aldrich
Hexanal, 98%
Supelco
Toluene, analytical standard
Supelco
Residual Solvent - Toluene, Pharmaceutical Secondary Standard; Certified Reference Material
Supelco
Ethyl acetate, analytical standard
Supelco
Benzaldehyde, Pharmaceutical Secondary Standard; Certified Reference Material
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Ethyl acetate, puriss., meets analytical specification of Ph. Eur., BP, NF, ≥99.5% (GC)
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Butyl acetate, ACS reagent, ≥99.5%
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Isobutyraldehyde, ≥99%
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Isovaleraldehyde, 97%
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Octanal, 99%
Sigma-Aldrich
Ethyl butyrate, 99%
Supelco
Toluene, Pharmaceutical Secondary Standard; Certified Reference Material
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Methacrolein, 95%
Supelco
Benzaldehyde, analytical standard
Sigma-Aldrich
Isobutyraldehyde, ≥98%, FG
Sigma-Aldrich
Ethyl propionate, 99%
Supelco
Ethyl Acetate, Pharmaceutical Secondary Standard; Certified Reference Material

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