• Home
  • Search Results
  • Determination of cefaclor by UPLC-MS-MS for a Chinese pharmacokinetic study.

Determination of cefaclor by UPLC-MS-MS for a Chinese pharmacokinetic study.

Journal of chromatographic science (2013-07-11)
Chenrong Huang, Wei Wang, Liyan Miao
ABSTRACT

A novel method has been developed for the determination of cefaclor in human plasma by ultra-performance liquid chromatography combined with tandem mass spectrometry (UPLC-MS-MS). The plasma was treated by a single step of protein precipitation with acetonitrile. The chromatographic separation was performed on a Waters Acquity UPLC BEH C18 (2.1 × 100 mm, 1.7 µm) with a gradient mobile phase consisting of 0.1% formic acid and acetonitrile at a flow rate of 0.4 mL/min. The analyses were conducted by multiple reaction monitoring using the precursor-to-product combinations of m/z 367.5 → 173.8 (cefaclor) and m/z 454.1 → 160.3 (internal standard). Validation results indicated that the lower limit of quantification was 2 ng/mL and the assay exhibited a linear range of 2-10,000 ng/mL. Quality control samples (5, 200 and 5,000 ng/mL) in five replicates from three different runs of analysis demonstrated an intra-assay precision (relative standard deviation) of 3.7-10.7%, an inter-assay precision of 5.8-8.9%, and an overall accuracy of < 15%. A sensitive and specific method for quantifying cefaclor in human plasma has been devised and successfully applied to a pharmacokinetic study.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Acetonitrile, suitable for HPLC, gradient grade, ≥99.9%
Sigma-Aldrich
Acetonitrile, anhydrous, 99.8%
Sigma-Aldrich
Acetonitrile, HPLC Plus, ≥99.9%
Sigma-Aldrich
Acetonitrile, suitable for HPLC, gradient grade, ≥99.9%
Sigma-Aldrich
Acetonitrile solution, contains 0.1 % (v/v) trifluoroacetic acid, suitable for HPLC
Sigma-Aldrich
Acetonitrile, ACS reagent, ≥99.5%
Sigma-Aldrich
Acetonitrile, suitable for HPLC-GC, ≥99.8% (GC)
Sigma-Aldrich
Acetonitrile solution, contains 0.1 % (v/v) formic acid, suitable for HPLC
Sigma-Aldrich
Acetonitrile, HPLC Plus, ≥99.9%, poly-coated bottles
Supelco
Acetonitrile, Pharmaceutical Secondary Standard; Certified Reference Material
Supelco
Acetonitrile, analytical standard
Sigma-Aldrich
Acetonitrile, biotech. grade, ≥99.93%
Sigma-Aldrich
Acetonitrile, ReagentPlus®, 99%
Sigma-Aldrich
Acetonitrile, electronic grade, 99.999% trace metals basis
Sigma-Aldrich
Cefaclor
Supelco
Residual Solvent - Acetonitrile, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
Acetonitrile, suitable for DNA synthesis, ≥99.9% (GC)
Sigma-Aldrich
Acetonitrile solution, contains 0.05 % (w/v) ammonium formate, 0.1 % (v/v) formic acid, 5 % (v/v) water, suitable for HPLC
Sigma-Aldrich
Acetonitrile, puriss. p.a., ACS reagent, reag. Ph. Eur., ≥99.5% (GC)
Supelco
Cefaclor, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
Acetonitrile, ≥99.5%, ACS reagent
Sigma-Aldrich
Acetonitrile solution, contains 10.0% acetone, 40.0% 2-propanol, 0.05% formic acid
USP
Residual Solvent Class 2 - Acetonitrile, United States Pharmacopeia (USP) Reference Standard
Sigma-Aldrich
Acetonitrile solution, contains 0.035 % (v/v) trifluoroacetic acid, suitable for HPLC
Sigma-Aldrich
Acetonitrile solution, contains 0.05 % (v/v) trifluoroacetic acid
USP
Cefaclor, United States Pharmacopeia (USP) Reference Standard
Sigma-Aldrich
Acetonitrile
Supelco
Acetonitrile, HPLC grade, ≥99.93%