MilliporeSigma
  • Home
  • Search Results
  • Gastric cancer-derived MSC-secreted PDGF-DD promotes gastric cancer progression.

Gastric cancer-derived MSC-secreted PDGF-DD promotes gastric cancer progression.

Journal of cancer research and clinical oncology (2014-06-19)
Feng Huang, Mei Wang, Tingting Yang, Jie Cai, Qiang Zhang, Zixuan Sun, Xiaodan Wu, Xu Zhang, Wei Zhu, Hui Qian, Wenrong Xu
ABSTRACT

This study was designed to investigate the role of PDGF-DD secreted by gastric cancer-derived mesenchymal stem cells (GC-MSCs) in human gastric cancer progression. Gastric cancer cells were indirectly co-cultured with GC-MSCs in a transwell system. The growth and migration of gastric cancer cells were evaluated by cell colony formation assay and transwell migration assay, respectively. The production of PDGF-DD in GC-MSCs was determined by using Luminex and ELISA. Neutralization of PDGFR-β by su16f and siRNA interference of PDGF-DD in GC-MSCs was used to demonstrate the role of PDGF-DD produced by GC-MSCs in gastric cancer progression. GC-MSC conditioned medium promoted gastric cancer cell proliferation and migration in vitro and in vivo. Co-culture with GC-MSCs increased the phosphorylation of PDGFR-β in SGC-7901 cells. Neutralization of PDGFR-β by su16f blocked the promoting role of GC-MSC conditioned medium in gastric cancer cell proliferation and migration. Recombinant PDGF-DD duplicated the effects of GC-MSC conditioned medium on gastric cancer cells. Knockdown of PDGF-DD in GC-MSCs abolished its effects on gastric cancer cells in vitro and in vivo. PDGF-DD secreted by GC-MSCs is capable of promoting gastric cancer cell progression in vitro and in vivo. Targeting the PDGF-DD/PDGFR-β interaction between MSCs and gastric cancer cells may represent a novel strategy for gastric cancer therapy.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Monoclonal Anti-PDGFRB antibody produced in mouse, clone 4C12, purified immunoglobulin, buffered aqueous solution
Sigma-Aldrich
Anti-PDGFRB antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Sigma-Aldrich
Anti-VIM antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Sigma-Aldrich
Anti-Bcl-2 (ab1) antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
Sigma-Aldrich
Monoclonal Anti-CD140b antibody produced in mouse, clone 18A2, purified immunoglobulin, buffered aqueous solution
Sigma-Aldrich
MISSION® esiRNA, targeting human AKR1C2
Sigma-Aldrich
MISSION® esiRNA, targeting human ATP2A2
Sigma-Aldrich
Anti-CDH1 antibody produced in goat, affinity isolated antibody, buffered aqueous solution
Sigma-Aldrich
MONOCLONAL ANTI-PDGFR antibody produced in mouse, clone 275CT1, crude ascites, buffered aqueous solution
Sigma-Aldrich
Anti-Cdh1 antibody,Mouse monoclonal, clone DCS-266, purified from hybridoma cell culture
Sigma-Aldrich
Monoclonal Anti-Vimentin antibody produced in mouse, clone VIM-13.2, ascites fluid
Sigma-Aldrich
Anti-Vimentin antibody produced in goat, whole antiserum
Sigma-Aldrich
Monoclonal Anti-Vimentin antibody produced in mouse, clone LN-6, ascites fluid
Sigma-Aldrich
Monoclonal Anti-Bcl-2 antibody produced in mouse, clone Bcl-2-100, ascites fluid
Sigma-Aldrich
Dimethyl sulfoxide, ≥99.5% (GC), suitable for plant cell culture
Sigma-Aldrich
Dimethyl sulfoxide, for molecular biology
Sigma-Aldrich
Dimethyl sulfoxide, Hybri-Max, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%
Sigma-Aldrich
Dimethyl sulfoxide, meets EP testing specifications, meets USP testing specifications
Sigma-Aldrich
Dimethyl sulfoxide, PCR Reagent
Sigma-Aldrich
Anti-Vimentin antibody, Mouse monoclonal, clone V9, purified from hybridoma cell culture
Sigma-Aldrich
Dimethyl sulfoxide, BioUltra, for molecular biology, ≥99.5% (GC)
Sigma-Aldrich
Dimethyl sulfoxide, sterile-filtered, BioPerformance Certified, meets EP, USP testing specifications, suitable for hybridoma
Sigma-Aldrich
Monoclonal Anti-VIM antibody produced in mouse, clone 3E9, purified immunoglobulin, buffered aqueous solution
Sigma-Aldrich
Anti-CDH1 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Sigma-Aldrich
Dimethyl sulfoxide solution, 50 wt. % in H2O
Sigma-Aldrich
Monoclonal Anti-CDH1 antibody produced in mouse, clone 3F4, purified immunoglobulin, buffered aqueous solution
Sigma-Aldrich
Anti-Vimentin antibody produced in rabbit, affinity isolated antibody
Sigma-Aldrich
Monoclonal Anti-VIM antibody produced in mouse, clone VI-RE/1, purified immunoglobulin, buffered aqueous solution
Sigma-Aldrich
Anti-BCL2 antibody produced in rabbit, affinity isolated antibody
Sigma-Aldrich
Monoclonal Anti-CDH1 antibody produced in mouse, clone 67A4, purified immunoglobulin, buffered aqueous solution