A new ELISA for quantification of brassinosteroids in plants.

Steroids (2014-09-10)
Andrey G Pradko, Raisa P Litvinovskaya, Alina L Sauchuk, Svetlana V Drach, Alexander V Baranovsky, Vladimir N Zhabinskii, Tatyana V Mirantsova, Vladimir A Khripach
ABSTRACT

Starting from (22R,23R)-2α,3α,22,23,26-pentahydroxy-5α-cholestan-6-one 26-hemisuccinate, conjugates of 28-norcastasterone with horse radish peroxidase and bovine serum albumin were prepared. The latter conjugate was injected into rabbits; produced polyclonal antibodies were used to quantitate 6-keto-brassinosteroids. The newly developed analytical system was used in combination with two other immunoenzymatic assays for brassinosteroids to determine individual compounds of this series. In addition, a direct method of brassinosteroid analysis was proposed. It has the advantage of requiring no sample pretreatment steps such as extraction with organic solvents and chromatography.

MATERIALS
Product Number
Brand
Product Description

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