MilliporeSigma
  • Home
  • Search Results
  • Rapid inactivation of Mycobacterium and nocardia species before identification using matrix-assisted laser desorption ionization-time of flight mass spectrometry.

Rapid inactivation of Mycobacterium and nocardia species before identification using matrix-assisted laser desorption ionization-time of flight mass spectrometry.

Journal of clinical microbiology (2014-08-01)
W Michael Dunne, Kirk Doing, Elizabeth Miller, Eric Miller, Erik Moreno, Mehdi Baghli, Sandrine Mailler, Victoria Girard, Alex van Belkum, Parampal Deol
ABSTRACT

The identification of mycobacteria outside biocontainment facilities requires that the organisms first be rendered inactive. Exposure to 70% ethanol (EtOH) either before or after mechanical disruption was evaluated in order to establish a safe, effective, and rapid inactivation protocol that is compatible with identification of Mycobacterium and Nocardia species using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). A combination of 5 min of bead beating in 70% EtOH followed by a 10-min room temperature incubation period was found to be rapidly bactericidal and provided high-quality spectra compared to spectra obtained directly from growth on solid media. The age of the culture, the stability of the refrigerated or frozen lysates, and freeze-thaw cycles did not adversely impact the quality of the spectra or the identification obtained.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Ammonium-14N2 sulfate solution, 40 wt. % in H2O, 99.99 atom % 14N
Sigma-Aldrich
Ammonium-14N2,sulfate-16O4, 99.99 atom % 14N, 99.99 atom % 16O
Sigma-Aldrich
Ammonium sulfate-14N2 solution, 40 wt. % in H2O, 99.99 atom % 14N
Sigma-Aldrich
Ammonium sulfate, suitable for plant cell culture, ≥99.0%
Sigma-Aldrich
Ammonium-14N2 sulfate, 99.99 atom % 14N
Sigma-Aldrich
Ammonium sulfate, for molecular biology, ≥99.0%
Sigma-Aldrich
Ammonium sulfate, BioUltra, ≥99.0% (T)
Sigma-Aldrich
Ammonium sulfate, BioXtra, ≥99.0%
Sigma-Aldrich
Ammonium sulfate, 99.999% trace metals basis
Supelco
Ammonium sulfate, analytical standard, for Nitrogen Determination According to Kjeldahl Method, traceable to NIST SRM 194
Sigma-Aldrich
Ammonium sulfate, anhydrous, free-flowing, Redi-Dri, ACS reagent, ≥99.0%
Sigma-Aldrich
Ammonium sulfate, anhydrous, Redi-Dri, ReagentPlus®, ≥99.0%
Sigma-Aldrich
Ammonium sulfate, ACS reagent, ≥99.0%
Sigma-Aldrich
Ammonium sulfate, ReagentPlus®, ≥99.0%