• Home
  • Search Results
  • Serum autoantibodies in pristane induced lupus are regulated by neutrophil gelatinase associated lipocalin.

Serum autoantibodies in pristane induced lupus are regulated by neutrophil gelatinase associated lipocalin.

Clinical immunology (Orlando, Fla.) (2014-06-28)
Rahul D Pawar, Beatrice Goilav, Yumin Xia, Haoyang Zhuang, Leal Herlitz, Westley H Reeves, Chaim Putterman
ABSTRACT

The onset of autoantibodies in systemic autoimmunity can be the result of a breakdown in tolerance at multiple checkpoints. Genetic, hormonal, and immunological factors can combine with environmental influences to accelerate the onset of disease and aggravate disease outcome. Here, we describe a novel mechanism relating to the regulatory role of Neutrophil Gelatinase Associated Lipocalin (NGAL) in modulating the levels of autoantibodies in pristane induced lupus. Following a single injection of pristane intraperitoneally, NGAL expression was induced in both the serum and spleen. Furthermore, NGAL deficient mice were more susceptible to the induction of pristane stimulated autoimmunity, and displayed higher numbers of autoantibody secreting cells and increased expression of activation induced cytidine deaminase (AID) and other inflammatory mediators in the spleen. In contrast, kidney damage was milder in NGAL deficient mice, indicating that NGAL was detrimental in autoantibody mediated kidney disease. These studies indicate that NGAL plays differential roles in different tissues in the context of lupus, and suggest a previously unrecognized role for NGAL in adaptive immunity.

MATERIALS
Product Number
Brand
Product Description

Supelco
Hematoxylin
Sigma-Aldrich
4-Nitrophenyl phosphate disodium salt hexahydrate, suitable for enzyme immunoassay, ≥99.0% (enzymatic)
Sigma-Aldrich
Phosphatase substrate, 5 mg tablets
Sigma-Aldrich
Hematoxylin, certified by the Biological Stain Commission
Sigma-Aldrich
Pristane, synthetic, ≥98% (GC)
Sigma-Aldrich
Phosphatase substrate, powder
Sigma-Aldrich
4-Nitrophenyl phosphate disodium salt hexahydrate, tablet
Sigma-Aldrich
4-Nitrophenyl phosphate disodium salt hexahydrate, tablet
Sigma-Aldrich
4-Nitrophenyl phosphate disodium salt hexahydrate, powder, BioReagent, suitable for cell culture, ≥97%
SAFC
Pristane, synthetic, liquid, sterile-filtered, BioReagent
Sigma-Aldrich
4-Nitrophenyl phosphate disodium salt hexahydrate, tablet
Sigma-Aldrich
Human MCP-1 / CCL2 ELISA Kit, for serum, plasma, cell culture supernatant and urine
Sigma-Aldrich
Phosphatase substrate, 40 mg tablets
Sigma-Aldrich
Phosphatase substrate, 100 mg capsules
Sigma-Aldrich
Mouse MCP-1 / CCL2 ELISA Kit, for serum, plasma and cell culture supernatant
Sigma-Aldrich
Lipocalin-2 human, recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC)
Sigma-Aldrich
Phosphatase substrate, Suitable for manufacturing of diagnostic kits and reagents
Sigma-Aldrich
Phosphatase substrate, 40 mg capsules
Sigma-Aldrich
Rat MCP-1 / CCL2 ELISA Kit, for serum, plasma and cell culture supernatant
Sigma-Aldrich
Rat MCP-1 / CCL2 ELISA Kit, for cell and tissue lysates
Sigma-Aldrich
Mouse MCP-1 / CCL2 ELISA Kit, for cell and tissue lysates