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Role of flavonoids on oxidative stress and mineral contents in the retinoic acid-induced bone loss model of rat.

European journal of nutrition (2013-11-26)
Nada Oršolić, Eleonora Goluža, Domagoj Dikić, Duje Lisičić, Kristijan Sašilo, Edi Rođak, Zelko Jeleč, Maja Vihnanek Lazarus, Tatjana Orct
ABSTRACT

Reactive oxygen species play a role in a number of degenerative conditions including osteoporosis. Flavonoids as phyto-oestrogens exert physiological effects against oxidative stress diseases. We developed a retinoic acid-induced bone loss model of rats to assess whether flavonoids and alendronate as positive control have role against oxidative stress and mineral contents in osteoporosis in vivo. Three-month-old female rats of the Y59 strain were given quercetin, chrysin, naringenin (100 mg kg(-1)) or alendronate (40 mg kg(-1), a positive control) immediately before retinoic acid treatment (80 mg kg(-1)) once daily for 14 days by a single intragastric (i.g.) application. In the second part of the study, we assessed the effect of those flavonoids on the skeletal system of healthy rats using single i.g. application on the respective flavonoids during 14 days. Twenty-four hours after the treatment, we analysed bone mineral density and the total content of bone calcium and phosphorus in the femur, the geometric and physical characteristics of thigh bones and lipid peroxidation and glutathione levels of liver and kidney cells. All flavonoids improved the decrease in bone weight coefficient, the length and the diameter of the bone, the content of bone ash and calcium and phosphorus content induced by retinoic acid. Chrysin and quercetin showed promise as preventive agents. Flavonoids were superior to alendronate according to some criteria. These results suggest that the dietary flavonoids could reduce retinoic acid-induced oxidative stress and bone loss and that flavonoids may be useful therapeutics for prevention of skeletal diseases.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
(±)-Naringenin, ≥95%
Sigma-Aldrich
Chrysin, 97%
Supelco
(±)-Naringenin, analytical standard
Quercetin dihydrate, primary reference standard
Quercetin dihydrate, European Pharmacopoeia (EP) Reference Standard
Supelco
Chrysin, analytical standard
Sigma-Aldrich
Naringenin, natural (US), 98%