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Toward structured macroporous hydrogel composites: electron beam-initiated polymerization of layered cryogels.

Biomacromolecules (2015-03-03)
Anna Golunova, David Chvátil, Pavel Krist, Josef Jaroš, Veronika Jurtíková, Jakub Pospíšil, Ilya Kotelnikov, Lucie Abelová, Jiří Kotek, Tomáš Sedlačík, Jan Kučka, Jana Koubková, Hana Studenovská, Libor Streit, Aleš Hampl, František Rypáček, Vladimír Proks
ABSTRACT

The ability to tailor mechanical properties and architecture is crucial in creating macroporous hydrogel scaffolds for tissue engineering. In the present work, a technique for the modification of the pore size and stiffness of acrylamide-based cryogels is demonstrated via the regulation of an electron beam irradiation dose. The samples were characterized by equilibrium swelling measurements, light and scanning electron microscopy, mercury porosimetry, Brunauer-Emmett-Teller surface area analysis, and stiffness measurements. Their properties were compared to cryogels prepared by a standard redox-initiated radical polymerization. A (125)I radiolabeled azidopentanoyl-GGGRGDSGGGY-NH2 peptide was bound to the surface to determine the concentration of the adhesive sites available for biomimetic modification. The functionality of the prepared substrates was evaluated by in vitro cultivation of adipose-derived stem cells. Moreover, the feasibility of preparing layered cryogels was demonstrated. This may be the key to the future preparation of complex hydrogel-based scaffolds to mimic the extracellular microenvironment in a wide range of applications.

MATERIALS
Product Number
Brand
Product Description

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DAPI, for nucleic acid staining
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Copper(II) sulfate pentahydrate, ACS reagent, ≥98.0%
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Glutaraldehyde solution, Grade I, 25% in H2O, specially purified for use as an electron microscopy fixative
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Ethidium bromide solution, BioReagent, for molecular biology, 10 mg/mL in H2O
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Glutaraldehyde solution, 50 wt. % in H2O
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Glutaraldehyde solution, Grade I, 50% in H2O, specially purified for use as an electron microscopy fixative or other sophisticated use
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(+)-Sodium L-ascorbate, powder, BioReagent, suitable for cell culture
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(+)-Sodium L-ascorbate, BioXtra, ≥99.0% (NT)
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N,N′-Methylenebisacrylamide, powder, for molecular biology, suitable for electrophoresis, ≥99.5%
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Copper(II) sulfate pentahydrate, BioReagent, suitable for cell culture, ≥98%
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