MilliporeSigma
  • Home
  • Search Results
  • Creatinine metabolite, HMH (5-hydroxy-1-methylhydantoin; NZ-419), modulates bradykinin-induced changes in vascular smooth muscle cells.

Creatinine metabolite, HMH (5-hydroxy-1-methylhydantoin; NZ-419), modulates bradykinin-induced changes in vascular smooth muscle cells.

Journal of receptor and signal transduction research (2014-03-20)
Kazuharu Ienaga, Mimi Sohn, Mitsuru Naiki, Ayad A Jaffa
ABSTRACT

A creatinine metabolite, 5-hydroxy-1-methylhydantoin (HMH: NZ-419), a hydroxyl radical scavenger, has previously been shown to confer renoprotection by inhibiting the progression of chronic kidney disease in rats. In the current study, we demonstrate that HMH modulates the effects of glucose and bradykinin (BK) in vascular smooth muscle cell (VSMC). HMH a novel anti-oxidant drug completely suppressed the expression of B2-kinin receptors (B2KR) in response to high glucose (25 mM) stimulation in VSMC and was also shown to attenuate the effects of BK on VSMC remodeling. HMH inhibited the BK-induced increase in MAPK phosphorylation and attenuated the increase in connective tissue growth factor (CTGF) protein levels in VSMC. These findings suggest that HMH may confer vascular protection against high glucose concentrations and BK-stimulation to ameliorate vascular injury and remodeling through its anti-oxidant properties.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Monoclonal Anti-BDKRB2 antibody produced in mouse, clone 3F6, purified immunoglobulin, buffered aqueous solution
Sigma-Aldrich
Monoclonal Anti-PLEKHM1 antibody produced in mouse, clone 1C9, purified immunoglobulin, buffered aqueous solution
Sigma-Aldrich
Anti-MAPK14 antibody produced in rabbit, affinity isolated antibody
Sigma-Aldrich
Anti-JNK antibody, Mouse monoclonal, clone 1C2, purified from hybridoma cell culture
Sigma-Aldrich
Anti-p38 MAPK antibody produced in rabbit, affinity isolated antibody
Sigma-Aldrich
Anti-p38 MAPK antibody produced in rabbit, affinity isolated antibody
Sigma-Aldrich
Anti-p38 MAPK antibody produced in rabbit, affinity isolated antibody
Sigma-Aldrich
Anti-Synaptophysin antibody produced in rabbit, affinity isolated antibody
Sigma-Aldrich
Anti-p38 MAP Kinase antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution
Sigma-Aldrich
Monoclonal Anti-MAP Kinase 2 (ERK-2) antibody produced in mouse, clone 1B3B9, purified immunoglobulin, buffered aqueous solution
Sigma-Aldrich
DL-Tyrosine, 99%
Sigma-Aldrich
Anti-PLEKHM1 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution, Ab2
Sigma-Aldrich
Anti-PLEKHM1 antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
Sigma-Aldrich
Monoclonal Anti-MAPK8 antibody produced in mouse, clone 2F11, purified immunoglobulin, buffered aqueous solution
Sigma-Aldrich
Anti-BDKRB2 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Sigma-Aldrich
Anti-Synaptophysin antibody, Mouse monoclonal, clone SVP-38, purified from hybridoma cell culture
Tyrosine, European Pharmacopoeia (EP) Reference Standard
Sigma-Aldrich
Monoclonal Anti-Synaptophysin antibody produced in mouse, clone SVP-38, ascites fluid
Sigma-Aldrich
Anti-MAP Kinase 2 (ERK-2) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution, ~1 mg/mL
Sigma-Aldrich
Anti-SYP antibody produced in rabbit, affinity isolated antibody
Sigma-Aldrich
Anti-JNK1 antibody produced in rabbit, affinity isolated antibody
Sigma-Aldrich
Anti-SYP antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Sigma-Aldrich
Anti-MAPK14 (Ab-182) antibody produced in rabbit, affinity isolated antibody
Sigma-Aldrich
Anti-MAPK8 antibody produced in mouse, purified immunoglobulin, buffered aqueous solution
Sigma-Aldrich
Anti-MAPK14 antibody produced in mouse, purified immunoglobulin, buffered aqueous solution