MilliporeSigma
  • Home
  • Search Results
  • Folding graft copolymer with pendant drug segments for co-delivery of anticancer drugs.

Folding graft copolymer with pendant drug segments for co-delivery of anticancer drugs.

Biomaterials (2014-05-31)
Wanyi Tai, Ran Mo, Yue Lu, Tianyue Jiang, Zhen Gu
ABSTRACT

A graft copolymer with pendant drug segments can fold into nanostructures in a protein folding-like manner. The graft copolymer is constructed by directly polymerizing γ-camptothecin-glutamate N-carboxyanhydride (Glu(CPT)-NCA) on multiple sites of poly(ethylene glycol) (PEG)-based main chain via the ring open polymerization (ROP). The "purely" conjugated anticancer agent camptothecin (CPT) is hydrophobic and serves as the principal driving force during the folding process. When exposed to water, the obtained copolymer, together with doxorubicin (Dox), another anticancer agent, can fold into monodispersed nanocarriers (with a diameter of around 50 nm) for dual-drug delivery. Equipped with a PEG shell, the nanocarriers displayed good stability and can be internalized by a variety of cancer cell lines via the lipid raft and clathrin-mediated endocytotic pathway without premature leakage, which showed a high synergetic activity of CPT and Dox toward various cancer cells. In vivo study validated that the nanocarriers exhibited strong accumulation in tumor sites and showed a prominent anticancer activity against the lung cancer xenograft mice model compared with free drugs.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
4-(Dimethylamino)pyridine, ReagentPlus®, ≥99%
Sigma-Aldrich
Triphosgene, reagent grade, 98%
Sigma-Aldrich
DIC, 99%
Sigma-Aldrich
N-Hydroxysuccinimide, 98%
Sigma-Aldrich
Di-tert-butyl dicarbonate solution, 1.0 M in THF
Sigma-Aldrich
4-(Dimethylamino)pyridine, purum, ≥98.0% (NT)
Supelco
Trifluoroacetic acid, analytical standard
Sigma-Aldrich
DIC, purum, ≥98.0% (GC)
Sigma-Aldrich
N-Hydroxysuccinimide, purum, ≥97.0% (T)
Sigma-Aldrich
Di-tert-butyl dicarbonate solution, 2 M in methylene chloride
Sigma-Aldrich
Di-tert-butyl dicarbonate solution, 2 M in THF
Sigma-Aldrich
Trifluoroacetic acid, puriss. p.a., suitable for HPLC, ≥99.0% (GC)
Sigma-Aldrich
Trifluoroacetic acid, ≥99%, for protein sequencing
Sigma-Aldrich
Trifluoroacetic acid, suitable for HPLC, ≥99.0%
Sigma-Aldrich
Trifluoroacetic acid, ReagentPlus®, 99%
Sigma-Aldrich
Diisopropylcarbodiimide solution, 1 M in dichloromethane
Sigma-Aldrich
Diisopropylcarbodiimide solution, 1 M in THF
Supelco
Acetamide, analytical standard
Valaciclovir impurity G, European Pharmacopoeia (EP) Reference Standard
Supelco
Triethylamine, analytical standard
USP
Valacyclovir Related Compound G, United States Pharmacopeia (USP) Reference Standard
Sigma-Aldrich
Acetamide, sublimed, 99%
Sigma-Aldrich
Di-tert-butyl dicarbonate, ReagentPlus®, 99%
Sigma-Aldrich
Di-tert-butyl dicarbonate, ReagentPlus®, ≥99%
Sigma-Aldrich
Tris(2-aminoethyl)amine, 96%
Sigma-Aldrich
Triethylamine, ≥99.5%
Sigma-Aldrich
4-(Dimethylamino)pyridine solution, 0.5 M in THF
Sigma-Aldrich
Triethylamine, ≥99%
Sigma-Aldrich
Acetamide, ~99% (GC)
Sigma-Aldrich
Boc-Glu-OtBu