MilliporeSigma
  • Home
  • Search Results
  • Pinus densiflora Sieb. et Zucc. alleviates lipogenesis and oxidative stress during oleic acid-induced steatosis in HepG2 cells.

Pinus densiflora Sieb. et Zucc. alleviates lipogenesis and oxidative stress during oleic acid-induced steatosis in HepG2 cells.

Nutrients (2014-07-25)
Yu-Jin Hwang, Hae-Ri Wi, Haeng-Ran Kim, Kye Won Park, Kyung-A Hwang
ABSTRACT

Excess accumulation of lipids and oxidative stress in the liver contribute to nonalcoholic fatty liver disease (NAFLD). We hypothesized that Pinus densiflora Sieb. et Zucc. (PSZ) can protect against NAFLD by regulating lipid accumulation and oxidative stress in the liver. To investigate the effect of PSZ upon NAFLD, we used an established cellular model: HepG2 cells treated with oleic acid. Then, the extent of hepatic steatosis and oxidative stress was assessed and levels of inflammatory markers measured. Oleic acid-treated HepG2 cells, compared with controls, had greater lipid accumulation. PSZ decreased lipid accumulation by 63% in oleic acid-treated HepG2 cells. Additionally, PSZ decreased the target gene expression of lipogenesis such as sterol regulatory element binding protein-1c, fatty acid synthase, stearoyl-CoA desaturase-1, diacylglycerol O-acyltransferase-1, and acetyl-CoA carboxylase-1 by 1.75, 6.0, 2.32, 1.93 and 1.81 fold, respectively. In addition, Oleic acid-treated HepG2 cells elicited extensive accumulation of tumor necrosis factor-α (TNFα) by 4.53 fold, whereas PSZ-treated cells decreased the expression of TNFα mRNA by 1.76 fold. PSZ significantly inhibited oxidative stress induced by reactive oxygen species. These results suggest that PSZ has effects on steatosis in vitro and further studies are needed in vivo to verify the current observations.

MATERIALS
Product Number
Brand
Product Description

Boron, monofilament, 20m, diameter 0.2mm
Boron, monofilament, 20m, diameter 0.1mm
Boron, monofilament, 10m, diameter 0.2mm
Boron, microfoil, disks, 10mm, thinness 0.1μm, specific density 23.5μg/cm2, permanent mylar 3.5μm support, 99.6%
Boron, monofilament, 5m, diameter 0.1mm
Boron, monofilament, 200m, diameter 0.1mm
Boron, monofilament, 50m, diameter 0.2mm
Boron, monofilament, 5m, diameter 0.2mm
Boron, monofilament, 50m, diameter 0.1mm
Boron, monofilament, 100m, diameter 0.1mm
Boron, monofilament, 10m, diameter 0.1mm
Boron, microfoil, disks, 25mm, thinness 0.1μm, specific density 23.5μg/cm2, permanent mylar 3.5μm support, 99.6%
Sigma-Aldrich
Boron, crystalline, −60 mesh, 99% trace metals basis
Sigma-Aldrich
Boron, ≥95% (boron), amorphous powder
Sigma-Aldrich
Boron, crystalline, 1 cm, 99.7% trace metals basis
Sigma-Aldrich
Oleic acid, suitable for cell culture, BioReagent
Supelco
Oleic acid, analytical standard
Sigma-Aldrich
Oleic acid, meets analytical specification of Ph, Eur., 65.0-88.0% (GC)
Sigma-Aldrich
Oleic acid, technical grade, 90%
Sigma-Aldrich
Oleic acid, ≥99% (GC)
Sigma-Aldrich
Oleic acid, natural, FCC
Supelco
Oleic acid, Selectophore, ≥99.0%
Oleic acid, European Pharmacopoeia (EP) Reference Standard