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E. coli MG1655 modulates its phospholipid composition through the cell cycle.

FEBS letters (2015-08-15)
Samuel Furse, Hans Wienk, Rolf Boelens, Anton I P M de Kroon, J Antoinette Killian
ABSTRACT

This paper describes a study of the phospholipid profile of Escherichia coli MG1655 cultures at the B and D periods of the cell cycle. The results indicate that the phosphatidyl glycerol fraction grows relatively rapidly and that the size of the cardiolipin (CL) fraction does not grow at all during cell elongation. This is consistent with observations that CL is located preferentially at the poles of E. coli. It also suggests that lipid production is controlled as a function of the cell cycle.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
LB Broth (Lennox), Highly-referenced microbial growth powder medium, low salt, suitable for salt-sensitive E.coli culture.
Sigma-Aldrich
N,N-Dimethylformamide-d7, ≥99.5 atom % D, contains 0.03 % (v/v) TMS