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  • The human core exosome interacts with differentially localized processive RNases: hDIS3 and hDIS3L.

The human core exosome interacts with differentially localized processive RNases: hDIS3 and hDIS3L.

The EMBO journal (2010-06-10)
Rafal Tomecki, Maiken S Kristiansen, Søren Lykke-Andersen, Aleksander Chlebowski, Katja M Larsen, Roman J Szczesny, Karolina Drazkowska, Agnieszka Pastula, Jens S Andersen, Piotr P Stepien, Andrzej Dziembowski, Torben Heick Jensen
ABSTRACT

The eukaryotic RNA exosome is a ribonucleolytic complex involved in RNA processing and turnover. It consists of a nine-subunit catalytically inert core that serves a structural function and participates in substrate recognition. Best defined in Saccharomyces cerevisiae, enzymatic activity comes from the associated subunits Dis3p (Rrp44p) and Rrp6p. The former is a nuclear and cytoplasmic RNase II/R-like enzyme, which possesses both processive exo- and endonuclease activities, whereas the latter is a distributive RNase D-like nuclear exonuclease. Although the exosome core is highly conserved, identity and arrangements of its catalytic subunits in different vertebrates remain elusive. Here, we demonstrate the association of two different Dis3p homologs--hDIS3 and hDIS3L--with the human exosome core. Interestingly, these factors display markedly different intracellular localizations: hDIS3 is mainly nuclear, whereas hDIS3L is strictly cytoplasmic. This compartmental distribution reflects the substrate preferences of the complex in vivo. Both hDIS3 and hDIS3L are active exonucleases; however, only hDIS3 has retained endonucleolytic activity. Our data suggest that three different ribonucleases can serve as catalytic subunits for the exosome in human cells.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Anti-DIS3 antibody, Mouse monoclonal, clone DIS3-N, purified from hybridoma cell culture
Sigma-Aldrich
Anti-EXOSC7 (C-terminal) antibody produced in rabbit, ~1.0 mg/mL, affinity isolated antibody
Sigma-Aldrich
Anti-EXOSC7 (N-terminal) antibody produced in rabbit, ~1.0 mg/mL, affinity isolated antibody
Sigma-Aldrich
Anti-DIS3 antibody, Mouse monoclonal, clone DIS3-3, purified from hybridoma cell culture
Sigma-Aldrich
Anti-EXOSC2 antibody produced in rabbit, ~1.0 mg/mL, affinity isolated antibody
Sigma-Aldrich
Anti-EXOSC4 antibody produced in rabbit, ~1 mg/mL, affinity isolated antibody
Sigma-Aldrich
Anti-DIS3 antibody produced in rabbit, ~1.0 mg/mL, affinity isolated antibody
Sigma-Aldrich
Anti-DIS3L antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Sigma-Aldrich
Anti-EXOSC2 antibody produced in rabbit, affinity isolated antibody
Sigma-Aldrich
Anti-EXOSC2 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Sigma-Aldrich
Monoclonal Anti-EXOSC2 antibody produced in mouse, clone 4B6, purified immunoglobulin, buffered aqueous solution

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