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Prelimbic Cortical Injections of a GABA Agonist and Antagonist: In Vivo Quantification of the Effect in the Rat Brain Using [(18)F] FDG MicroPET.

Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging (2015-04-29)
Joke Parthoens, Stijn Servaes, Jeroen Verhaeghe, Sigrid Stroobants, Steven Staelens
ABSTRACT

We evaluated the glucose metabolism after microinjections of a GABAA antagonist, bicuculline, and a GABAA agonist, muscimol, in the rat prelimbic cortex (PL) by small animal positron emission tomography (μPET). Following a microinjection of either 0.5 μl bicuculline (0.1 mg/ml), muscimol (1 mg/ml), or saline in the left PL of 11 healthy male Sprague Dawley rats (250-275 g), 2-deoxy-2-[(18)F]fluoro-β-D-glucose ([(18)F]FDG) PET images were acquired. Volume-of-interest (VOI)-based analysis and voxel-based statistical parametric mapping were performed (n = 9). VOI-based analysis revealed significantly different [(18)F]FDG uptake following bicuculline versus muscimol in PL (p < 0.001), infralimbic cortex (p < 0.01), and cingulate cortex (p < 0.01). Voxel-based analysis showed bicuculline induced widespread significant hypermetabolism throughout the brain while muscimol induced significant localized hypometabolism. Here, we visualize functional GABAA-mediated correlations of the PL following pharmacological stimulation. This could serve as a reference and shed light on the working and focality of other stimulation paradigms targeting this region.

MATERIALS
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Brand
Product Description

Sigma-Aldrich
2-Methylbutane, anhydrous, ≥99%
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2-Methylbutane, ReagentPlus®, ≥99%
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2-Methylbutane, puriss. p.a., ≥99.5% (GC)
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2-Methylbutane, ReagentPlus®, ≥99%
Sigma-Aldrich
Tantalum(V) ethoxide, 99.98% trace metals basis
Sigma-Aldrich
Nitrogen, ≥99.998%
Tantalum(V) ethoxide, packaged for use in deposition systems