Rapid Column-Free Enrichment of Mononuclear Cells from Solid Tissues.

Scientific reports (2015-08-01)
Steven D Scoville, Karen A Keller, Stephanie Cheng, Michael Zhang, Xiaoli Zhang, Michael A Caligiuri, Aharon G Freud
ABSTRACT

We have developed a rapid negative selection method to enrich rare mononuclear cells from human tissues. Unwanted and antibody-tethered cells are selectively depleted during a Ficoll separation step, and there is no need for magnetic-based reagents and equipment. The new method is fast, customizable, inexpensive, remarkably efficient, and easy to perform, and per sample the overall cost is less than one-tenth the cost associated with a magnetic column-based method.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Phorbol 12-myristate 13-acetate, ≥99% (TLC), film or powder
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Ammonium chloride, 99.998% trace metals basis
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Ethylenediaminetetraacetic acid, 99.995% trace metals basis
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Phorbol 12-myristate 13-acetate, synthetic, ≥98.0% (TLC)
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