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Biochemical conversion of sugarcane straw hemicellulosic hydrolyzate supplemented with co-substrates for xylitol production.

Bioresource technology (2015-12-01)
A F Hernández-Pérez, I A L Costa, D D V Silva, K J Dussán, T R Villela, E V Canettieri, J A Carvalho, T G Soares Neto, M G A Felipe
ABSTRACT

Biotechnological production of xylitol is an attractive route to add value to a sugarcane biorefinery, through utilization of the hemicellulosic fraction of sugarcane straw, whose availability is increasing in Brazil. Herein, supplementation of the sugarcane straw hemicellulosic hydrolyzate (xylose 57gL(-1)) with maltose, sucrose, cellobiose or glycerol was proposed, and their effect as co-substrates on xylitol production by Candida guilliermondii FTI 20037 was studied. Sucrose (10gL(-1)) and glycerol (0.7gL(-1)) supplementation led to significant increase of 8.88% and 6.86% on xylose uptake rate (1.11gL(-1)h(-1) and 1.09gL(-1)), respectively, but only with sucrose, significant increments of 12.88% and 8.69% on final xylitol concentration (36.11gL(-1)) and volumetric productivity (0.75gL(-1)h(-1)), respectively, were achieved. Based on these results, utilization of complex sources of sucrose, derived from agro-industries, as nutritional supplementation for xylitol production can be proposed as a strategy for improving the yeast performance and reducing the cost of this bioprocess by replacing more expensive nutrients.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
D-(+)-Xylose, ≥99%
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Ethyl alcohol, Pure, 190 proof, meets USP testing specifications
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Ethyl alcohol, Pure, 190 proof, ACS spectrophotometric grade, 95.0%
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Glycerol, BioUltra, for molecular biology, anhydrous, ≥99.5% (GC)
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Glycerol, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for electrophoresis, ≥99% (GC)
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5α-Androstan-17β-ol-3-one, ≥97.5%
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D-(+)-Xylose, BioUltra, ≥99.0% (sum of enantiomers, HPLC)
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Glycerol, ≥99.5%
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Glycerol, for molecular biology, ≥99.0%
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Glycerol, BioXtra, ≥99% (GC)
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D-(+)-Xylose, BioXtra, ≥99%
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Glycerol, FCC, FG
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D-(+)-Xylose, ≥99%
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Ethyl alcohol, Pure, 200 proof, anhydrous, ≥99.5%
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Glycerol, puriss., meets analytical specification of Ph. Eur., BP, USP, FCC, E422, anhydrous, 99.0-101.0% (alkalimetric)
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Ethyl alcohol, Pure, 200 proof, for molecular biology
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Ethyl alcohol, Pure, 190 proof, for molecular biology
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Glycerol, ReagentPlus®, ≥99.0% (GC)
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Glycerol, ACS reagent, ≥99.5%
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Ethyl alcohol, Pure, 200 proof, HPLC/spectrophotometric grade
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Glycerol, puriss. p.a., ACS reagent, anhydrous, dist., ≥99.5% (GC)
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Ethyl alcohol, Pure, 200 proof, ACS reagent, ≥99.5%
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Ethyl alcohol, Pure, 200 proof, meets USP testing specifications
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Ethanol, ACS reagent, prima fine spirit, without additive, F15 o1
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Sulfuric acid, ACS reagent, 95.0-98.0%
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Acetic acid, glacial, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.8%
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Acetic acid, glacial, puriss., meets analytical specification of Ph. Eur., BP, USP, FCC, 99.8-100.5%
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Acetic acid solution, suitable for HPLC
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