Ultrafast sonochemical synthesis of protein-inorganic nanoflowers.

International journal of nanomedicine (2015-09-09)
Bhagwan S Batule, Ki Soo Park, Moon Il Kim, Hyun Gyu Park
ABSTRACT

We developed a simple but efficient method to synthesize protein-inorganic hybrid nanostructures with a flower-like shape (nanoflowers), which relies on sonication to facilitate the synthesis of the nanoflowers. With this technique, we synthesized nanoflowers containing laccase as a model protein and copper phosphate within 5 minutes at room temperature. The resulting laccase nanoflowers yielded greatly enhanced activity, stability, and reusability, and their usefulness was successfully demonstrated by applying them in the colorimetric detection of epinephrine. The strategy developed could be used to rapidly synthesize nanoflowers for various applications in biosensor and enzyme catalysis and would expand the utilization of nanoflowers in diverse fields of biotechnology.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Copper(II) sulfate pentahydrate, ACS reagent, ≥98.0%
Sigma-Aldrich
Copper(II) sulfate pentahydrate, BioReagent, suitable for cell culture, ≥98%
Sigma-Aldrich
Copper(II) sulfate pentahydrate, ReagentPlus®, ≥98.0%
Sigma-Aldrich
Copper(II) sulfate pentahydrate, suitable for plant cell culture, ≥98%
Sigma-Aldrich
Copper(II) sulfate pentahydrate, 99.999% trace metals basis
Sigma-Aldrich
Copper(II) sulfate pentahydrate, puriss., suitable for, meets analytical specification of Ph. Eur., BP, USP,FCC
Sigma-Aldrich
Copper(II) sulfate pentahydrate, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., 99-102%
Sigma-Aldrich
Copper(II) sulfate pentahydrate, 99.995% trace metals basis
Sigma-Aldrich
Syringaldazine, indicator for laccase and peroxidase activity
Sigma-Aldrich
Syringaldazine, 98%