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Tumor suppressive microRNA-137 negatively regulates Musashi-1 and colorectal cancer progression.

Oncotarget (2015-05-06)
Amber R Smith, Rebecca T Marquez, Wei-Chung Tsao, Surajit Pathak, Alexandria Roy, Jie Ping, Bailey Wilkerson, Lan Lan, Wenjian Meng, Kristi L Neufeld, Xiao-Feng Sun, Liang Xu
ABSTRACT

Stem cell marker, Musashi-1 (MSI1) is over-expressed in many cancer types; however the molecular mechanisms involved in MSI1 over-expression are not well understood. We investigated the microRNA (miRNA) regulation of MSI1 and the implications this regulation plays in colorectal cancer. MicroRNA miR-137 was identified as a MSI1-targeting microRNA by immunoblotting and luciferase reporter assays. MSI1 protein was found to be highly expressed in 79% of primary rectal tumors (n=146), while miR-137 expression was decreased in 84% of the rectal tumor tissues (n=68) compared to paired normal mucosal samples. In addition to reduced MSI1 protein, exogenous expression of miR-137 inhibited cell growth, colony formation, and tumorsphere growth of colon cancer cells. Finally, in vivo studies demonstrated that induction of miR-137 can decrease growth of human colon cancer xenografts. Our results demonstrate that miR-137 acts as a tumor-suppressive miRNA in colorectal cancers and negatively regulates oncogenic MSI1.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Doxycycline hyclate
Sigma-Aldrich
Trioctylphosphine, technical grade, 90%
Sigma-Aldrich
Trioctylphosphine, 97%