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  • Pretreatment with lipopolysaccharide attenuates diethylnitrosamine-caused liver injury in mice via TLR4-dependent induction of Kupffer cell M2 polarization.

Pretreatment with lipopolysaccharide attenuates diethylnitrosamine-caused liver injury in mice via TLR4-dependent induction of Kupffer cell M2 polarization.

Immunologic research (2015-04-08)
Xianjing Li, Zhuo Wang, Yulian Zou, Ermei Lu, Jingjing Duan, Hongbao Yang, Qijin Wu, Xiaona Zhao, Yun Wang, Linjun You, Ling He, Tao Xi, Yong Yang
ABSTRACT

In this study, we found that pretreatment with low dose of lipopolysaccharide (LPS), also known as lipoglycans and endotoxin, obviously attenuated liver injury caused by diethylnitrosamine (DEN) in mice. This protective effect was described by decreased ALT, TNF-α, and IL-1β and increased TGF-β production. However, Toll-like receptor 4-deficient (TLR4(-/-)) or macrophages depletion abolished this protection in mice, which revealed Kupffer cells (KCs) and TLR4 to be crucial for the prevention of LPS against DEN-induced damage. Further study revealed that LPS pretreatment induced the KCs to M2 polarization and impaired the signaling of MAPKs and NF-κB that mediated the production of inflammatory cytokines. Moreover, T regulatory cells (Tregs) were also recruited to the liver, which may mediate immunosuppression and participate in the prevention of DEN-induced injury. Our results suggested that LPS protected against DEN-induced hepatitis via induction of M2 Kupffer cells and recruitment of Tregs, which contributes to liver tolerance in TLR4-dependent mechanism.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Gadolinium(III) chloride, anhydrous, powder, 99.99% trace metals basis
Sigma-Aldrich
DL-Alanine, ≥99% (HPLC)
Sigma-Aldrich
DL-Alanine, ≥99%, FCC, FG
Sigma-Aldrich
Gadolinium(III) chloride solution, NMR reference standard, 0.1 mg/mL in D2O (99.9 atom % D), water 1 %, Methanol-13C 0.1 % (99 atom % 13C), NMR tube size 3 mm × 8 in.
Supelco
Gadolinium(III) chloride solution, NMR reference standard, 0.1 mg/mL in D2O (99.9 atom % D), Methanol-13C 0.1 % (99 atom % 13C), water 1 %, NMR tube size 5 mm × 8 in.
Sigma-Aldrich
Gadolinium(III) chloride solution, NMR reference standard, 0.1 mg/mL in D2O (99.9 atom % D), water 0.1 %, NMR tube size 6.5 mm × 8 in.