Prospective isolation of human erythroid lineage-committed progenitors.

Proceedings of the National Academy of Sciences of the United States of America (2015-07-22)
Yasuo Mori, James Y Chen, John V Pluvinage, Jun Seita, Irving L Weissman
ABSTRACT

Determining the developmental pathway leading to erythrocytes and being able to isolate their progenitors are crucial to understanding and treating disorders of red cell imbalance such as anemia, myelodysplastic syndrome, and polycythemia vera. Here we show that the human erythrocyte progenitor (hEP) can be prospectively isolated from adult bone marrow. We found three subfractions that possessed different expression patterns of CD105 and CD71 within the previously defined human megakaryocyte/erythrocyte progenitor (hMEP; Lineage(-) CD34(+) CD38(+) IL-3Rα(-) CD45RA(-)) population. Both CD71(-) CD105(-) and CD71(+) CD105(-) MEPs, at least in vitro, still retained bipotency for the megakaryocyte (MegK) and erythrocyte (E) lineages, although the latter subpopulation is skewed in differentiation toward the erythroid lineage. Notably, the proliferative and differentiation output of the CD71(intermediate(int)/+) CD105(+) subset of cells within the MEP population was completely restricted to the erythroid lineage with the loss of MegK potential. CD71(+) CD105(-) MEPs are erythrocyte-biased MEPs (E-MEPs) and CD71(int/+) CD105(+) cells are EPs. These previously unclassified populations may facilitate further understanding of the molecular mechanisms governing human erythroid development and serve as potential therapeutic targets in disorders of the erythroid lineage.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Stem Cell Factor human, SCF, recombinant, expressed in E. coli, powder, suitable for cell culture
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Stem Cell Factor from mouse, SCF, recombinant, expressed in E. coli, powder, suitable for cell culture
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Stem Cell Factor human, SCF, recombinant, expressed in HEK 293 cells, suitable for cell culture
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SCF human, Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
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SCF from mouse, Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
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SCF from rat, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
Sigma-Aldrich
SCF from rat, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

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