Pseudoroseovarius zhejiangensis gen. nov., sp. nov., a novel alpha-proteobacterium isolated from the chemical wastewater, and reclassification of Roseovarius crassostreae as Pseudoroseovarius crassostreae comb. nov., Roseovarius sediminilitoris as Pseudoroseovarius sediminilitoris comb. nov. and Roseovarius halocynthiae as Pseudoroseovarius halocynthiae comb. nov.
A Gram-stain negative, aerobic, non-motile and ovoid- to rod-shaped bacterial strain, designated JB3(T), was isolated from a wastewater sample collected from the biochemical reaction basin of Haiyan fine chemical factory in Zhejiang, China. Strain JB3(T) was found to grow optimally at pH 7.0-8.0, at 28 °C and in the presence of 1.0-2.0 % (w/v) NaCl. Chemotaxonomic analysis showed that strain JB3(T) contains ubiquinone-10 (>99 %) as the predominant respiratory quinone and C18:1 ω7c (70.9 %) as the most abundant fatty acid. The polar lipids of strain JB3(T) were identified as phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, an aminophospholipid, an unidentified aminolipid, four unidentified phospholipids and three unidentified lipids. The DNA G+C content of strain JB3(T) was determined to be 68.1 mol%. The 16S rRNA gene sequence similarities between the isolate and Roseovarius crassostreae DSM 16950(T), Roseovarius sediminilitoris KCTC 23959(T) and Roseovarius halocynthiae MA1-10(T) were found to be 97.1, 96.8 and 96.2 %, respectively. Morevoer, the similarity between strain JB3(T) and the type strain of the genus Roseovarius (Roseovarius tolerans DSM 11457(T)) was found to be 93.8 %. The phylogenetic trees reconstructed with all three treeing methods showed that strain JB3(T) constituted a different taxon, which was separate from other taxa with validly published names, and formed a cluster with R. crassostreae DSM 16950(T), R. sediminilitoris KCTC 23959(T) and R. halocynthiae MA1-10(T). These three species were not placed within the phylogenetic cluster formed by R. tolerans DSM 11457(T). Differential phenotypic properties, together with the phylogenetic distinctiveness, demonstrated that strain JB3(T) is clearly distinct from species of the genus Roseovarius. On the basis of these features, we propose strain JB3(T) represents a novel species of a novel genus with the name Pseudoroseovarius zhejiangensis gen. nov., sp. nov. The type strain is JB3(T) (=MCCC 1K00457(T) = KCTC 42443(T)). We also propose that R. crassostreae, R. sediminilitoris and R. halocynthiae should be transferred to this new genus as Pseudoroseovarius crassostreae comb. nov., Pseudoroseovarius sediminilitoris comb. nov. and Pseudoroseovarius halocynthiae comb. nov., respectively.