MilliporeSigma
  • Home
  • Search Results
  • Are effects of common ragwort in the Ames test caused by pyrrolizidine alkaloids?

Are effects of common ragwort in the Ames test caused by pyrrolizidine alkaloids?

Mutation research (2015-05-30)
Toine F H Bovee, Richard J R Helsdingen, Ron L A P Hoogenboom, Monique W C M de Nijs, Xiaojie Liu, Klaas Vrieling, Peter G L Klinkhamer, Ad A C M Peijnenburg, Patrick P J Mulder
ABSTRACT

It has previously been demonstrated by others that acetone extracts of Senecio jacobaea (syn. Jacobaea vulgaris, common or tansy ragwort) test positive in the Salmonella/microsome mutagenicity test (Ames test). Pyrrolizidine alkaloids (PAs) are thought to be responsible for these mutagenic effects. However, it was also observed that the major PA present in common ragwort, jacobine, produced a negative response (with and without the addition of rat liver S9) in Salmonella test strains TA98, TA100, TA1535 and TA1537. To investigate which compounds in the plant extracts were responsible for the positive outcome, the present study investigated the contents and mutagenic effects of methanol and acetone extracts prepared from dried ground S. jacobaea and Senecio inaequidens (narrow-leafed ragwort). Subsequently, a fractionation approach was set up in combination with LC-MS/MS analysis of the fractions. It was shown that the positive Ames test outcomes of S. jacobaea extracts are unlikely to be caused by PAs, but rather by the flavonoid quercetin. This study also demonstrates the importance of identifying compounds responsible for positive test results in bioassays.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Citric acid monohydrate, ACS reagent, ≥99.0%
Sigma-Aldrich
Citric acid monohydrate, BioXtra, ≥99.5%
Sigma-Aldrich
Citric acid monohydrate, reagent grade, ≥98% (GC/titration)
Sigma-Aldrich
Potassium phosphate dibasic solution, 1.0 M
Sigma-Aldrich
Sodium phosphate monobasic, 99.998% trace metals basis
Sigma-Aldrich
2-Aminoanthracene, 96%
Sigma-Aldrich
Potassium phosphate dibasic, 99.95% trace metals basis
Sigma-Aldrich
β-Nicotinamide adenine dinucleotide phosphate hydrate
Sigma-Aldrich
Sodium phosphate monobasic, meets USP testing specifications, anhydrous
Sigma-Aldrich
Dextrose monohydrate, meets USP testing specifications
Sigma-Aldrich
Sodium phosphate monobasic, BioXtra, ≥99.0%
Sigma-Aldrich
Potassium phosphate dibasic, meets USP testing specifications
Sigma-Aldrich
Potassium phosphate dibasic, reagent grade, ≥98.0%
Sigma-Aldrich
Sodium phosphate monobasic, ReagentPlus®, ≥99.0%
Sigma-Aldrich
Sodium phosphate monobasic, BioReagent, for molecular biology, anhydrous, ≥98%
Sigma-Aldrich
Potassium phosphate dibasic, anhydrous, for luminescence, for molecular biology, BioUltra, ≥99.0% (T)
Sigma-Aldrich
Sodium phosphate monobasic, BioPerformance Certified, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.0% (titration)
Sigma-Aldrich
β-Nicotinamide adenine dinucleotide phosphate sodium salt, pkg of 5 mg (per vial)
Sigma-Aldrich
β-Nicotinamide adenine dinucleotide phosphate sodium salt, pkg of 10 mg (per vial)
Sigma-Aldrich
Sodium phosphate monobasic solution, BioUltra, 5 M in H2O
Sigma-Aldrich
Sodium phosphate monobasic, anhydrous, free-flowing, Redi-Dri, ≥99.0%
Sigma-Aldrich
Quercetin, ≥95% (HPLC), solid
Sigma-Aldrich
Sodium phosphate monobasic-16O4, 99.9 atom % 16O
Sigma-Aldrich
Magnesium sulfate heptahydrate, BioXtra, ≥99.0%
Sigma-Aldrich
Retrorsine, ≥90% (HPLC)
Sigma-Aldrich
Ammonium sodium phosphate dibasic tetrahydrate, BioXtra, ≥99%
Sigma-Aldrich
Ammonium sodium phosphate dibasic tetrahydrate, ≥99%
Sigma-Aldrich
Sodium phosphate dibasic dihydrate, BioUltra, for molecular biology, ≥99.0% (T)
Sigma-Aldrich
L-Histidine, BioUltra, ≥99.5% (NT)
Sigma-Aldrich
D-(+)-Glucose monohydrate, meets analytical specification of Ph. Eur., BP, Ph Franç., 7.0-9.5% water(Karl Fischer)