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Patterned Plasmonic Nanoparticle Arrays for Microfluidic and Multiplexed Biological Assays.

Analytical chemistry (2015-10-24)
Jie He, Michelle Boegli, Ian Bruzas, William Lum, Laura Sagle
ABSTRACT

For applications ranging from medical diagnostics and drug screening to chemical and biological warfare detection, inexpensive, rapid-readout, portable devices are required. Localized surface plasmon resonance (LSPR) technologies show substantial promise toward meeting these goals, but the generation of portable, multiplexed and/or microfluidic devices incorporating sensitive nanoparticle arrays is only in its infancy. Herein, we have combined photolithography with Hole Mask Colloidal lithography to pattern uniform nanoparticle arrays for both microfluidic and multiplexed devices. The first proof-of-concept study is carried out with 5- and 7-channel microfluidic devices to acquire one-shot binding curves and protein binding kinetic data. The second proof-of-concept study involved the fabrication of a 96-spot plate that can be inserted into a standard plate reader for the multiplexed detection of protein binding. This versatile fabrication technique should prove useful in next generation chips for bioassays and genetic screening.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Glycerol, for molecular biology, ≥99.0%
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Ammonium fluoride, ACS reagent, ≥98.0%
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Hydrofluoric acid, 48 wt. % in H2O, ≥99.99% trace metals basis
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Trichloro(octadecyl)silane, ≥90%
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Glycerol, BioUltra, for molecular biology, anhydrous, ≥99.5% (GC)
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Glycerol, puriss. p.a., ACS reagent, anhydrous, dist., ≥99.5% (GC)
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11-Mercaptoundecanoic acid, 98%
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Glycerol solution, puriss., meets analytical specification of Ph. Eur., BP, 84-88%
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