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The coffee-machine bacteriome: biodiversity and colonisation of the wasted coffee tray leach.

Scientific reports (2015-11-26)
Cristina Vilanova, Alba Iglesias, Manuel Porcar
ABSTRACT

Microbial communities are ubiquitous in both natural and artificial environments. However, microbial diversity is usually reduced under strong selection pressures, such as those present in habitats rich in recalcitrant or toxic compounds displaying antimicrobial properties. Caffeine is a natural alkaloid present in coffee, tea and soft drinks with well-known antibacterial properties. Here we present the first systematic analysis of coffee machine-associated bacteria. We sampled the coffee waste reservoir of ten different Nespresso machines and conducted a dynamic monitoring of the colonization process in a new machine. Our results reveal the existence of a varied bacterial community in all the machines sampled, and a rapid colonisation process of the coffee leach. The community developed from a pioneering pool of enterobacteria and other opportunistic taxa to a mature but still highly variable microbiome rich in coffee-adapted bacteria. The bacterial communities described here, for the first time, are potential drivers of biotechnologically relevant processes including decaffeination and bioremediation.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
2-Propanol, BioReagent, for molecular biology, ≥99.5%
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Sodium dodecyl sulfate, BioReagent, suitable for electrophoresis, for molecular biology, ≥98.5% (GC)
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Sodium chloride, BioXtra, ≥99.5% (AT)
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2-Propanol, anhydrous, 99.5%
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Sodium dodecyl sulfate, ACS reagent, ≥99.0%
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Potassium phosphate monobasic, ACS reagent, ≥99.0%
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Sodium chloride solution, 0.9% in water, BioXtra, suitable for cell culture
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Sucrose, ≥99.5% (GC)
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Sucrose, BioXtra, ≥99.5% (GC)
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Sodium dodecyl sulfate, ReagentPlus®, ≥98.5% (GC)
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2-Propanol, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.8% (GC)
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Isopropyl alcohol, ≥99.7%, FCC, FG
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Sucrose, BioUltra, for molecular biology, ≥99.5% (HPLC)
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Sodium dodecyl sulfate solution, BioUltra, for molecular biology, 10% in H2O
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Sucrose, ≥99.5%
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Potassium phosphate monobasic, ReagentPlus®
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Sucrose, puriss., meets analytical specification of Ph. Eur., BP, NF
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Sodium dodecyl sulfate, ≥99.0% (GC), dust-free pellets
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Isopropyl alcohol, meets USP testing specifications
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Sucrose, ACS reagent