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Myelinated mouse nerves studied by X-ray phase contrast zoom tomography.

Journal of structural biology (2015-11-08)
M Bartels, M Krenkel, P Cloetens, W Möbius, T Salditt
ABSTRACT

We have used X-ray phase contrast tomography to resolve the structure of uncut, entire myelinated optic, saphenous and sciatic mouse nerves. Intrinsic electron density contrast suffices to identify axonal structures. Specific myelin labeling by an osmium tetroxide stain enables distinction between axon and surrounding myelin sheath. Utilization of spherical wave illumination enables zooming capabilities which enable imaging of entire sciatic internodes as well as identification of sub-structures such as nodes of Ranvier and Schmidt-Lanterman incisures.

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