Enhanced Achilles tendon healing by fibromodulin gene transfer.

Nanomedicine : nanotechnology, biology, and medicine (2015-06-07)
Anthony Delalande, Marie-Pierre Gosselin, Arnaud Suwalski, William Guilmain, Chloé Leduc, Mathieu Berchel, Paul-Alain Jaffrès, Patrick Baril, Patrick Midoux, Chantal Pichon
ABSTRACT

Tendon injury is a major musculoskeletal disorder with a high public health impact. We propose a non-viral based strategy of gene therapy for the treatment of tendon injuries using histidylated vectors. Gene delivery of fibromodulin, a proteoglycan involved in collagen assembly was found to promote rat Achilles tendon repair in vivo and in vitro. In vivo liposome-based transfection of fibromodulin led to a better healing after surgical injury, biomechanical properties were better restored compared to untransfected control. These measures were confirmed by histological observations and scoring. To get better understandings of the mechanisms underlying fibromodulin transfection, an in vitro tendon healing model was developed. In vitro, polymer-based transfection of fibromodulin led to the best wound enclosure speed and a pronounced migration of tenocytes primary cultures was observed. These results suggest that fibromodulin non-viral gene therapy could be proposed as a new therapeutic strategy to accelerate tendon healing. Tendon injury is relatively common and healing remains unsatisfactory. In this study, the effects of liposomal-based delivery of fibromodulin gene were investigated in a rat Achilles tendon injury model. The positive results observed would provide a new therapeutic strategy in clinical setting in the future.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
2-Propanol, BioReagent, for molecular biology, ≥99.5%
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2-Propanol, ACS reagent, ≥99.5%
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Sodium dodecyl sulfate, BioReagent, suitable for electrophoresis, for molecular biology, ≥98.5% (GC)
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L-Ascorbic acid, 99%
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2-Propanol, anhydrous, 99.5%
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Sodium dodecyl sulfate, ACS reagent, ≥99.0%
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L-Ascorbic acid, powder, suitable for cell culture, γ-irradiated
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Sodium dodecyl sulfate, ReagentPlus®, ≥98.5% (GC)
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2-Propanol, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.8% (GC)
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Isopropyl alcohol, ≥99.7%, FCC, FG
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L-Ascorbic acid, BioXtra, ≥99.0%, crystalline
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Sodium dodecyl sulfate solution, BioUltra, for molecular biology, 10% in H2O
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L-Ascorbic acid, suitable for cell culture, suitable for plant cell culture, ≥98%
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L-Ascorbic acid, ACS reagent, ≥99%
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Sodium dodecyl sulfate, ≥99.0% (GC), dust-free pellets
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Isopropyl alcohol, meets USP testing specifications
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Sodium dodecyl sulfate, BioUltra, for molecular biology, ≥99.0% (GC)
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2-Propanol, BioUltra, for molecular biology, ≥99.5% (GC)
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Sodium dodecyl sulfate solution, BioUltra, for molecular biology, 20% in H2O
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2-Propanol, puriss. p.a., ACS reagent, ≥99.8% (GC)
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Sodium dodecyl sulfate, BioXtra, ≥99.0% (GC)
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Sodium dodecyl sulfate, 92.5-100.5% based on total alkyl sulfate content basis
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2-Propanol, puriss., meets analytical specification of Ph. Eur., BP, USP, ≥99.5% (GC)
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L-Ascorbic acid, reagent grade, crystalline
Supelco
Sodium dodecyl sulfate, dust-free pellets, suitable for electrophoresis, for molecular biology, ≥99.0% (GC)
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2-Propanol, Laboratory Reagent, ≥99.5%
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L-Ascorbic acid, puriss. p.a., ACS reagent, reag. ISO, Ph. Eur., 99.7-100.5% (oxidimetric)
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L-Ascorbic acid, analytical standard
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2-Propanol, electronic grade, 99.999% trace metals basis
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Sodium dodecyl sulfate, ≥90%