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Scaffold-free three-dimensional culture systems for mass production of periosteum-derived progenitor cells.

Journal of bioscience and bioengineering (2015-02-03)
Hyun-Myoung Cha, Sun-Mi Kim, Yong-Soo Choi, Dong-Il Kim
ABSTRACT

Mesenchymal stem cells (MSCs) are capable of self-renewal and can differentiate into various types of cells for therapeutic purposes. MSCs are frequently cultured in a two-dimensional (2D) system. However, MSCs can lose their differentiation capacity over time in this culture system. In addition, the available surface area for the propagation of cells is limited. Therefore, various three-dimensional (3D) culture systems have been developed. In this study, we developed the scaffold-free 3D culture systems for the expansion of periosteum-derived progenitor cells (PDPCs) as spheres. The spheroid formation of PDPCs was induced using a rotation platform. The spheres maintained their viability and proliferation ability. Moreover, expression levels of the stemness marker genes and proteins were higher in cells grown on 3D culture system than in 2D culture system. In conclusion, a simple and economical 3D culture system has been developed that can increase the potential of PDPCs for clinical use.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Propidium iodide, ≥94.0% (HPLC)
Sigma-Aldrich
Propidium iodide solution, solution (1.0 mg/ml in water)
Sigma-Aldrich
Calcein-AM, BioReagent, suitable for fluorescence, ≥95.0% (HPLC)
Sigma-Aldrich
Calcein AM solution, 4 mM in DMSO, ≥90% (HPLC), solution
Sigma-Aldrich
Sodium thiosulfate, ≥99.99% trace metals basis
Sigma-Aldrich
Calcein-AM, Small Package (20 X 50 μg ), ≥95.0% (HPLC)
Sigma-Aldrich
Sodium thiosulfate solution, 2 g/dL in deionized water