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Effect of Surface Chemical Functionalities on Collagen Deposition by Primary Human Dermal Fibroblasts.

ACS applied materials & interfaces (2015-10-13)
Akash Bachhuka, John Hayball, Louise E Smith, K Vasilev

Surface modification has been identified as an important technique that could improve the response of the body to implanted medical devices. Collagen production by fibroblasts is known to play a vital role in wound healing and device fibrous encapsulation. However, how surface chemistry affects collagen I and III deposition by these cells has not been systematically studied. Here, we report how surface chemistry influences the deposition of collagen I and III by primary human dermal fibroblasts. Amine (NH3), carboxyl acid (COOH), and hydrocarbon (CH3) surfaces were generated by plasma deposition. This is a practically relevant tool to deposit a functional coating on any type of substrate material. We show that fibroblasts adhere better and proliferate faster on amine-rich surfaces. In addition, the initial collagen I and III production is greater on this type of coating. These data indicates that surface modification can be a promising route for modulating the rate and level of fibrous encapsulation and may be useful in informing the design of implantable biomedical devices to produce more predictable clinical outcomes.

Product Number
Product Description

DAPI, for nucleic acid staining
Acrylic acid, anhydrous, contains 200 ppm MEHQ as inhibitor, 99%
Allylamine, 98%
Allylamine, ≥99%

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