• Home
  • Search Results
  • (13)C-metabolic flux analysis in S-adenosyl-L-methionine production by Saccharomyces cerevisiae.

(13)C-metabolic flux analysis in S-adenosyl-L-methionine production by Saccharomyces cerevisiae.

Journal of bioscience and bioengineering (2015-04-29)
Kenshi Hayakawa, Shuichi Kajihata, Fumio Matsuda, Hiroshi Shimizu
ABSTRACT

S-Adenosyl-L-methionine (SAM) is a major biological methyl group donor, and is used as a nutritional supplement and prescription drug. Yeast is used for the industrial production of SAM owing to its high intracellular SAM concentrations. To determine the regulation mechanisms responsible for such high SAM production, (13)C-metabolic flux analysis ((13)C-MFA) was conducted to compare the flux distributions in the central metabolism between Kyokai no. 6 (high SAM-producing) and S288C (control) strains. (13)C-MFA showed that the levels of tricarboxylic acid (TCA) cycle flux in SAM-overproducing strain were considerably increased compared to those in the S228C strain. Analysis of ATP balance also showed that a larger amount of excess ATP was produced in the Kyokai 6 strain because of increased oxidative phosphorylation. These results suggest that high SAM production in Kyokai 6 strains could be attributed to enhanced ATP regeneration with high TCA cycle fluxes and respiration activity. Thus, maintaining high respiration efficiency during cultivation is important for improving SAM production.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Glycine, suitable for electrophoresis, ≥99%
Sigma-Aldrich
Glycine, ReagentPlus®, ≥99% (HPLC)
Sigma-Aldrich
Potassium phosphate dibasic, ACS reagent, ≥98%
Sigma-Aldrich
Ammonium sulfate, for molecular biology, ≥99.0%
Sigma-Aldrich
Ammonium sulfate, ACS reagent, ≥99.0%
Sigma-Aldrich
Glycine, BioUltra, for molecular biology, ≥99.0% (NT)
Sigma-Aldrich
Potassium phosphate dibasic, reagent grade, ≥98.0%
Sigma-Aldrich
Glycine, ACS reagent, ≥98.5%
Sigma-Aldrich
Glycine, from non-animal source, meets EP, JP, USP testing specifications, suitable for cell culture, ≥98.5%
Sigma-Aldrich
Ammonium sulfate, ReagentPlus®, ≥99.0%
Sigma-Aldrich
Ammonium sulfate, BioXtra, ≥99.0%
Sigma-Aldrich
DL-Methionine, ≥99%
Sigma-Aldrich
Potassium phosphate dibasic, anhydrous, for luminescence, for molecular biology, BioUltra, ≥99.0% (T)
Sigma-Aldrich
Potassium phosphate dibasic, meets USP testing specifications
Sigma-Aldrich
Potassium phosphate dibasic solution, 1.0 M
SAFC
Glycine
Sigma-Aldrich
N-tert-Butyldimethylsilyl-N-methyltrifluoroacetamide with 1% tert-Butyldimethylchlorosilane, ≥95%
Sigma-Aldrich
Nitrogen, ≥99.998%
Sigma-Aldrich
Ammonium sulfate, BioUltra, ≥99.0% (T)
Sigma-Aldrich
Glycine, BioXtra, ≥99% (titration)
Sigma-Aldrich
Potassium phosphate dibasic, puriss. p.a., ACS reagent, anhydrous, ≥99.0% (T)
Sigma-Aldrich
Ammonium sulfate, 99.999% trace metals basis
Sigma-Aldrich
DL-Alanine, ≥99% (HPLC)
Sigma-Aldrich
Glycine, 99%, FCC
Sigma-Aldrich
DL-Methionine, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥99%
Sigma-Aldrich
Ammonium sulfate, suitable for plant cell culture, ≥99.0%
Sigma-Aldrich
DL-Alanine, ≥99%, FCC, FG
Sigma-Aldrich
Potassium phosphate dibasic, 99.95% trace metals basis
Sigma-Aldrich
DL-Methionine, 99%, FCC, FG
Sigma-Aldrich
Ammonium sulfate, anhydrous, free-flowing, Redi-Dri, ACS reagent, ≥99.0%