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Quantitative Characterization of Magnetic Mobility of Nanoparticle in Solution-Based Condition.

Current pharmaceutical design (2015-09-18)
Didem Rodoplu, Ismail H Boyaci, Akif G Bozkurt, Haslet Eksi, Adem Zengin, Ugur Tamer, Nihal Aydogan, Sadan Ozcan, Fatmanur Tugcu-Demiröz
ABSTRACT

Magnetic nanoparticles are considered as the ideal substrate to selectively isolate target molecules or organisms from sample solutions in a wide variety of applications including bioassays, bioimaging and environmental chemistry. The broad array of these applications in fields requires the accurate magnetic characterization of nanoparticles for a variety of solution based-conditions. Because the freshly synthesized magnetic nanoparticles demonstrated a perfect magnetization value in solid form, they exhibited a different magnetic behavior in solution. Here, we present simple quantitative method for the measurement of magnetic mobility of nanoparticles in solution-based condition. Magnetic mobility of the nanoparticles was quantified with initial mobility of the particles using UV-vis absorbance spectroscopy in water, ethanol and MES buffer. We demonstrated the efficacy of this method through a systematic characterization of four different core-shell structures magnetic nanoparticles over three different surface modifications. The solid nanoparticles were characterized using transmission electron microscopy (TEM), X-ray diffraction (XRD) and saturation magnetization (Ms). The surfaces of the nanoparticles were functionalized with 11-mercaptoundecanoic acid and bovine serum albumin BSA was selected as biomaterial. The effect of the surface modification and solution media on the stability of the nanoparticles was monitored by zeta potentials and hydrodynamic diameters of the nanoparticles. Results obtained from the mobility experiments indicate that the initial mobility was altered with solution media, surface functionalization, size and shape of the magnetic nanoparticle. The proposed method easily determines the interactions between the magnetic nanoparticles and their surrounding biological media, the magnetophoretic responsiveness of nanoparticles and the initial mobilities of the nanoparticles.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
2-Mercaptoethanol, ≥99.0%
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2-Mercaptoethanol, for molecular biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)
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Sodium chloride, BioXtra, ≥99.5% (AT)
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N-Hydroxysuccinimide, 98%
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Perchloric acid, ACS reagent, 70%
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Ethylenediaminetetraacetic acid, ACS reagent, 99.4-100.6%, powder
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Sodium chloride, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%
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Sodium chloride solution, 0.9% in water, BioXtra, suitable for cell culture
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MES hydrate, ≥99.5% (titration)
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Ethanolamine, ≥98%
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Sodium chloride solution, 5 M in H2O, BioReagent, for molecular biology, suitable for cell culture
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Ethylenediaminetetraacetic acid, anhydrous, BioUltra, ≥99% (titration)
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Sodium chloride solution, 5 M
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Trisodium citrate dihydrate, meets USP testing specifications
SAFC
Sodium chloride solution, 5 M
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Ethanolamine, ACS reagent, ≥99.0%
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1,2-Dichloroethane, ACS reagent, ≥99.0%
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2-Mercaptoethanol, BioUltra, for molecular biology, ≥99.0% (GC)
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Sodium citrate tribasic dihydrate, for molecular biology, ≥99%
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