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Evaluation of Mycelial Nutrients, Bioactive Compounds, and Antioxidants of Five Himalayan Entomopathogenic Ascomyceteous Fungi from India.

International journal of medicinal mushrooms (2015-11-13)
Sapan Kumar Sharma, Nandini Gautam, Narender Singh Atri
ABSTRACT

In this study, using standard methods, mycelial nutrients, bioactive compounds, and antioxidants were analyzed for the first time for five fungal species: Isaria sinclairii (Berk.) Lloyd, I. tenuipes Peck, I. japonica Yasuda, I. farinosa (Holmsk) Fr. and Cordyceps tuberculata (Lebert) Maire. All of these species were low in fat content and rich in protein, fiber, ash, and carbohydrates. Mineral elements (Fe, Mg, Cu, Mn, and Ca) were detected in appreciable amounts. All three types of fatty acids (saturated, monounsaturated, and polyunsaturated) as well as bioactive compounds (ascorbic acid, β-carotene, lycopene, phenolic compounds, and polysaccharides) were detected for each species. The investigated species showed high ferric-reducing antioxidant power as well as 2,2-diphenyl-1-picryl-hydrazyl radical scavenging activity. Although differences were observed in the values of each species, each species showed richness in one or more components.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Acetone, ACS reagent, ≥99.5%
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Hexane, anhydrous, 95%
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Hexane, ReagentPlus®, ≥99%
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Acetone, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.5% (GC)
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Hexane, Laboratory Reagent, ≥95%
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Hexane, puriss. p.a., ACS reagent, reag. Ph. Eur., ≥99% (GC)
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Acetone, Laboratory Reagent, ≥99.5%
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Acetone, suitable for HPLC, ≥99.9%
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Acetone, puriss., meets analytical specification of Ph. Eur., BP, NF, ≥99% (GC)
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Acetone, ACS reagent, ≥99.5%
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Acetone, histological grade, ≥99.5%
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Acetone solution, certified reference material, 2000 μg/mL in methanol: water (9:1)
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Acetone, ≥99%, meets FCC analytical specifications
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Hexane, HPLC Plus, for HPLC, GC, and residue analysis, ≥95%
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Acetone solution, contains 20.0 % (v/v) acetonitrile, suitable for HPLC
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Hexane, suitable for HPLC, ≥95%