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Fiber type-specific expression of low-density lipoprotein receptor-related protein 6 in human skeletal muscles.

Pathobiology : journal of immunopathology, molecular and cellular biology (2014-01-25)
Ikuko Takeda, Tetsuya Takahashi, Kazuhide Ochi, Takashi Kurashige, Yukari Shinozaki, Masahiro Nakamori, Koji Arihiro, Hirofumi Maruyama, Masayasu Matsumoto
ABSTRACT

Gene expression patterns differ in the two types of skeletal muscle fiber. The Wnt signaling pathway, which includes low-density lipoprotein receptor-related protein 6 (LRP6), has been associated with cell differentiation and glucose metabolism in skeletal muscles. We examined the relationships between muscle fiber types and LRP6 expression. Adenosine triphosphatase was assayed histochemically, and the levels of expression of LRP6 and myosin were analyzed immunohistochemically, in frozen sections of muscle fiber obtained from 16 muscle biopsy samples. The expression pattern of LRP6 in C2C12 cells was assayed by immunocytochemistry. LRP6 was expressed only in type II fibers. Type IIc fibers showed variations in LRP6 expression. Expression of LRP6 was observed at the stage of myoblast differentiation. Antibody to LRP6 may be useful for identifying type II skeletal muscle fibers. LRP6 may influence glucose metabolism in type II fibers of human skeletal muscles.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Hispidulin, ≥98% (HPLC)
Sigma-Aldrich
Anti-DVL2 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Sigma-Aldrich
Anti-LRP6 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Sigma-Aldrich
Anti-DVL3 (C-terminal region) antibody produced in rabbit, ~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

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