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Prenatal ketamine exposure causes abnormal development of prefrontal cortex in rat.

Scientific reports (2016-05-27)
Tianyun Zhao, Chuanxiang Li, Wei Wei, Haixing Zhang, Daqing Ma, Xingrong Song, Libing Zhou
ABSTRACT

Ketamine is commonly used for anesthesia and as a recreational drug. In pregnant users, a potential neurotoxicity in offspring has been noted. Our previous work demonstrated that ketamine exposure of pregnant rats induces affective disorders and cognitive impairments in offspring. As the prefrontal cortex (PFC) is critically involved in emotional and cognitive processes, here we studied whether maternal ketamine exposure influences the development of the PFC in offspring. Pregnant rats on gestational day 14 were treated with ketamine at a sedative dose for 2 hrs, and pups were studied at postnatal day 0 (P0) or P30. We found that maternal ketamine exposure resulted in cell apoptosis and neuronal loss in fetal brain. Upon ketamine exposure in utero, PFC neurons at P30 showed more dendritic branching, while cultured neurons from P0 PFC extended shorter neurites than controls. In addition, maternal ketamine exposure postponed the switch of NR2B/2A expression, and perturbed pre- and postsynaptic protein expression in the PFC. These data suggest that prenatal ketamine exposure impairs neuronal development of the PFC, which may be associated with abnormal behavior in offsprings.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Anti-Post Synaptic Density Protein 95 Antibody, clone 6G6-1C9, clone 6G6-1C9, Chemicon®, from mouse
Sigma-Aldrich
Anti-NR2B Antibody, Upstate®, from rabbit