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  • Screening of antioxidant activity and volatile compounds composition of Chamerion angustifolium (L.) Holub ecotypes grown in Lithuania.

Screening of antioxidant activity and volatile compounds composition of Chamerion angustifolium (L.) Holub ecotypes grown in Lithuania.

Natural product research (2015-07-30)
Vilma Kaškonienė, Audrius Maruška, Ieva Akuņeca, Mantas Stankevičius, Ona Ragažinskienė, Violeta Bartkuvienė, Olga Kornyšova, Vitalis Briedis, Rasa Ugenskienė
ABSTRACT

Since biological activity of medicinal plants is dependent on cultivation area, climatic conditions, developmental stage, genetic modifications and other factors, it is important to study flora present in different growing sites and geographical zones. This study was focused on screening of antioxidant activity of C. angustifolium harvested in six different locations in Lithuania. The total contents of phenolic compounds, flavonoids and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity were evaluated by spectrophotometric methods. A correlation between radical scavenging activity and total phenolic compounds content was observed (correlation coefficient 0.98). HPLC with online post-column DPPH radical scavenging reaction detection was used for the separation of extracts. Oenothein B, rutin and one unidentified compound were predominant. Volatile compounds were analysed using solid-phase microextraction coupled with gas chromatography-mass spectrometry. Based on the analysis of volatiles, all samples were classified into two chemotypes: (I) with predominant α- and β-caryophyllenes and (II) with predominant anethole.

MATERIALS
Product Number
Brand
Product Description

Supelco
SPME fiber assembly polyacrylate (PA), df 85 μm (PA), for use with autosampler, needle size 23 ga
Supelco
SPME fiber assembly Polydimethylsiloxane (PDMS), df 100 μm (PDMS), for use with manual holder, needle size 23 ga
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SPME fiber assembly Polydimethylsiloxane (PDMS), df 7 μm (PDMS), needle size 23 ga, for use with autosampler
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SPME Sampling Stand, for use with 15 mL vials
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SPME fiber assembly polyacrylate (PA), df 85 μm (PA), for use with autosampler, needle size 24 ga
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SPME Sampling Stand, for use with 4 mL vials
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SPME fiber assembly Polydimethylsiloxane (PDMS), df 30 μm (PDMS), needle size 24 ga, for use with autosampler
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SPME fiber assembly Polydimethylsiloxane (PDMS), df 100 μm (PDMS), for use with autosampler, needle size 23 ga
Supelco
SPME fiber assembly Polydimethylsiloxane (PDMS), df 100 μm (PDMS), for use with autosampler, needle size 24 ga
Supelco
SPME fiber assembly Polydimethylsiloxane (PDMS), df 7 μm (PDMS), needle size 24 ga, for use with manual holder
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SPME fiber assembly polyacrylate (PA), df 85 μm (PA), for use with manual holder, needle size 24 ga
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SPME Portable Field Sampler, coating CAR/PDMS
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SPME fiber assembly Polydimethylsiloxane (PDMS), df 7 μm (PDMS), needle size 24 ga, for use with autosampler
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SPME fiber assembly Polydimethylsiloxane (PDMS), df 30 μm (PDMS), needle size 24 ga, for use with manual holder
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SPME fiber assembly Polydimethylsiloxane (PDMS), df 100 μm (PDMS), needle size 24 ga, for use with manual holder
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SPME Portable Field Sampler, coating PDMS/DVB
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SPME Portable Field Sampler, coating PDMS
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SPME LC Tips, PDMS/DVB, pkg of 96 ea
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SPME-OC Fiber Assembly, Polydimethylsiloxane/Divinylbenzene (PDMS/DVB), df 75 μm (Coating thickness includes 65 μm coating + 10 μm OC (overcoating)), needle size 23 ga, pkg of 3 ea, fiber L 1 cm
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IonSense® C18 SPE-it Tips, pkg of 96 ea
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SPME fiber assembly Polydimethylsiloxane (PDMS), metal alloy fiber, df 100 μm (PDMS), needle size 23 ga, for use with autosampler
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IonSense® PDMS/DVB SPE-it Tips, pkg of 96 ea
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SPME fiber assortment kit 5, needle size 23 ga, for use with autosampler
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SPME fiber assortment kit 2, for use with manual holder, needle size 24 ga
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SPME fiber assembly, Carbowax-Polyethylene Glycol (PEG) Coating, needle size 23 ga, df 60 μm (PEG), for use with autosampler
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SPME fiber assembly, Carbowax-Polyethylene Glycol (PEG) Coating, needle size 23 ga, df 60 μm (PEG), metal alloy fiber, for use with manual holder
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SPME fiber assortment kit 2, for use with autosampler, needle size 23 ga
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SPME StableFlex fiber assortment kit, needle size 23 ga, for use with autosampler
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SPME fiber assortment kit 4, for use with autosampler, needle size 23 ga
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SPME fiber assortment kit 1, for use with autosampler, needle size 24 ga