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Dual specificity antibodies using a double-stranded oligonucleotide bridge.

FEBS letters (1999-06-01)
Z N Chaudri, M Bartlet-Jones, G Panayotou, T Klonisch, I M Roitt, T Lund, P J Delves
ABSTRACT

The covalent conjugation of oligonucleotides to antibody Fab' fragments was optimized by using oligonucleotides modified with a hexaethylene linker arm bearing three amino groups. One oligonucleotide was coupled to antibody of one specificity and a complementary oligonucleotide to antibody of a second specificity. The antibodies were then allowed to hybridize by base pairing of the complementary nucleotide sequences and the generation of bispecific antibody was analyzed on SDS-PAGE and confirmed using BIAcore analysis. The strategy of complementary oligonucleotide-linked bispecific molecules is not limited to antibodies but is applicable to linking any two molecules of different characteristics.

MATERIALS
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Product Description

Sigma-Aldrich
Anti-Human IgG (γ-chain specific), F(ab′)2 fragment antibody produced in goat, affinity isolated antibody, lyophilized powder

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