• Home
  • Search Results
  • Investigation of TbfA in Riemerella anatipestifer using plasmid-based methods for gene over-expression and knockdown.

Investigation of TbfA in Riemerella anatipestifer using plasmid-based methods for gene over-expression and knockdown.

Scientific reports (2016-11-16)
MaFeng Liu, MengYi Wang, DeKang Zhu, MingShu Wang, RenYong Jia, Shun Chen, KunFeng Sun, Qiao Yang, Ying Wu, XiaoYue Chen, Francis Biville, AnChun Cheng
ABSTRACT

Riemerella anatipestifer is a duck pathogen that has caused serious economic losses to the duck industry worldwide. Despite this, there are few reported studies of the physiological and pathogenic mechanisms of Riemerella anatipestifer infection. In previous study, we have shown that TonB1 and TonB2 were involved in hemin uptake. TonB family protein (TbfA) was not investigated, since knockout of this gene was not successful at that time. Here, we used a plasmid based gene over-expression and knockdown to investigate its function. First, we constructed three Escherichia-Riemerella anatipestifer shuttle vectors containing three different native Riemerella anatipestifer promoters. The shuttle plasmids were introduced into Riemerella anatipestifer ATCC11845 by conjugation at an efficiency of 5 × 10

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Lysozyme from chicken egg white, lyophilized powder, protein ≥90 %, ≥40,000 units/mg protein
Sigma-Aldrich
LB Broth (Miller), Highly-referenced nutrient-rich microbial growth powder medium, suitable for regular E.coli culture

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.