Cornea organoids from human induced pluripotent stem cells.

Scientific reports (2017-01-28)
James W Foster, Karl Wahlin, Sheila M Adams, David E Birk, Donald J Zack, Shukti Chakravarti
ABSTRACT

The cornea is the transparent outermost surface of the eye, consisting of a stratified epithelium, a collagenous stroma and an innermost single-cell layered endothelium and providing 2/3 of the refractive power of the eye. Multiple diseases of the cornea arise from genetic defects where the ultimate phenotype can be influenced by cross talk between the cell types and the extracellular matrix. Cell culture modeling of diseases can benefit from cornea organoids that include multiple corneal cell types and extracellular matrices. Here we present human iPS cell-derived organoids through sequential rounds of differentiation programs. These organoids share features of the developing cornea, harboring three distinct cell types with expression of key epithelial, stromal and endothelial cell markers. Cornea organoid cultures provide a powerful 3D model system for investigating corneal developmental processes and their disruptions in diseased conditions.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Retinoic acid, ≥98% (HPLC), powder
Sigma-Aldrich
ITS Liquid Media Supplement (100×), liquid, sterile-filtered, BioReagent, suitable for cell culture
Sigma-Aldrich
holo-Transferrin human, powder, BioReagent, suitable for cell culture, ≥97%