Surface De-PEGylation Controls Nanoparticle-Mediated siRNA Delivery

Theranostics (2017-06-24)
Xi Zhu, Wei Tao, Danny Liu, Jun Wu, Zilei Guo, Xiaoyuan Ji, Zameer Bharwani, Lili Zhao, Xiaoping Zhao, Omid C Farokhzad, Jinjun Shi
ABSTRACT

The present work proposes a unique de-PEGylation strategy for controllable delivery of small interfering RNA (siRNA) using a robust lipid-polymer hybrid nanoparticle (NP) platform. The self-assembled hybrid NPs are composed of a lipid-poly(ethylene glycol) (lipid-PEG) shell and a polymer/cationic lipid solid core, wherein the lipid-PEG molecules can gradually dissociate from NP surface in the presence of serum albumin. The de-PEGylation kinetics of a series of different lipid-PEGs is measured with their respective NPs, and the NP performance is comprehensively investigated

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
1,2-Distearoyl-sn-glycero-3-phosphoethanolamine, ≥99%
Sigma-Aldrich
1,2-Dipalmitoyl-sn-glycerol, ≥99%
Sigma-Aldrich
1,2-Dimyristoyl-sn-glycerol, ≥95%
Sigma-Aldrich
1,2-Dimyristoyl-sn-glycero-3-phosphoethanolamine, synthetic, ≥99%