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Characterization of Annexin V Fusion with the Superfolder GFP in Liposomes Binding and Apoptosis Detection.

Frontiers in physiology (2017-06-06)
Abdul Qader Abbady, Aya Twair, Bouthaina Ali, Hossam Murad
ABSTRACT

Programed cell death is a critical and unavoidable part of life. One of the most widely used markers for dying cells, by apoptosis or pyroptosis, is the redistribution of phosphatidylserine (PS) from the inner to the outer plasma membrane leaflet. Annexin V protein is a sensitive and specific probe to mark this event because of its high affinity to the exposed PS. Beyond that, annexin V can bind to any PS-containing phospholipid bilayer of almost all tiny forms of membranous vesicles like blood platelets, exosomes, or even nanostructured liposomes. In this work, recombinant human annexin V was produced as a fusion with a highly fluorescent superfolder derivative of the green fluorescent protein (

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
6-[Fluorescein-5(6)-carboxamido]hexanoic acid N-hydroxysuccinimide ester, suitable for fluorescence, ≥75% (HPLC)
Sigma-Aldrich
Annexin V human, recombinant, expressed in E. coli, GST tagged, ≥95% (SDS-PAGE)

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