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Immunomodulatory activity of recombinant α-gliadin conjugated to cholera toxin in DQ8 transgenic mice.

Immunology letters (2017-05-18)
Stefano Rossi, Diomira Luongo, Francesco Maurano, Paolo Bergamo, Mauro Rossi
ABSTRACT

Coeliac disease (CD) is characterized by an intestinal lesion sustained by an abnormal mucosal T-cell response to wheat gliadin. An immunological approach that is able to suppress this immune response is a perspective worth pursuing. Several strategies of antigen administration have been aimed at the downregulation of pathogenic T-cells. In particular, we previously reported a significant suppression of the systemic cell-mediated response toward wheat gliadin in DQ8 transgenic mice receiving nasally a recombinant α-gliadin. To gain further insight about the cellular mechanisms underlying the tolerogenic properties of this molecule, we analysed different preparations of the recombinant α-gliadin, alone or conjugated to the adjuvant cholera toxin (CT), by in vitro challenge with spleen CD4

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
3-(2-Pyridyldithio)propionic acid N-hydroxysuccinimide ester, ≥95%, powder
Sigma-Aldrich
Cholera Toxin from Vibrio cholerae, ≥90% (SDS-PAGE), lyophilized powder