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Rapid Throughput Analysis of GABA

Molecular pharmacology (2017-04-22)
Atefeh Mousavi Nik, Brandon Pressly, Vikrant Singh, Shane Antrobus, Susan Hulsizer, Michael A Rogawski, Heike Wulff, Isaac N Pessah
ABSTRACT

Fluorometric imaging plate reader membrane potential dye (FMP-Red-Dye) is a proprietary tool for basic discovery and high-throughput drug screening for G-protein-coupled receptors and ion channels. We optimized and validated this potentiometric probe to assay functional modulators of heterologous expressed GABA

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
trans-Androsterone
Supelco
Androsterone, VETRANAL®, analytical standard
Sigma-Aldrich
trans-Dehydroandrosterone, ≥99%
Sigma-Aldrich
5α-Pregnan-3α-ol-20-one, solid