Light is an attractive trigger for release of active molecules from nanocarriers in biological systems. Here, we describe a phenomenon of light-induced release of a fluorescent dye from lipid nano-droplets under visible light conditions. Using auto-emulsification process we prepared nanoemulsion droplets of 32nm size encapsulating the hydrophobic analogue of Nile Red, NR668. While these nano-droplets cannot spontaneously enter the cells on the time scale of hours, after illumination for 30s under the microscope at the wavelength of NR668 absorption (535nm), the dye showed fast accumulation inside the cells. The same phenomenon was observed in zebrafish, where nano-droplets initially staining the blood circulation were released into endothelial cells and tissues after illumination. Fluorescence correlation spectroscopy revealed that laser illumination at relatively low power (60mW/cm
Research. Development. Production.
We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.