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Standardisation of oxygen exposure in the development of mouse models for bronchopulmonary dysplasia.

Disease models & mechanisms (2017-01-10)
Claudio Nardiello, Ivana Mižíková, Diogo M Silva, Jordi Ruiz-Camp, Konstantin Mayer, István Vadász, Susanne Herold, Werner Seeger, Rory E Morty
ABSTRACT

Progress in developing new therapies for bronchopulmonary dysplasia (BPD) is sometimes complicated by the lack of a standardised animal model. Our objective was to develop a robust hyperoxia-based mouse model of BPD that recapitulated the pathological perturbations to lung structure noted in infants with BPD. Newborn mouse pups were exposed to a varying fraction of oxygen in the inspired air (FiO

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Product Description

Sigma-Aldrich
HEPES solution, 1 M, pH 7.0-7.6, sterile-filtered, BioReagent, suitable for cell culture
Sigma-Aldrich
Paraformaldehyde, reagent grade, crystalline
Millipore
Agar, suitable for microbiology
Sigma-Aldrich
Cottonseed oil