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Acetylated bovine serum albumin differentially inhibits polymerase chain reaction in microdevices.

Biomicrofluidics (2017-06-15)
Naveen Ramalingam, Majid Ebrahimi Warkiani, Thomas Hai-Qing Gong
ABSTRACT

Bovine serum albumin (BSA) is widely used as an additive in polymerase chain reaction (PCR)-based microfluidic devices to passivate reactors and alleviate nucleic-acid amplification. BSA is available commercially in two types: either acetylated or non-acetylated. A survey of literature indicates that both types of BSA are used in PCR-based microfluidic devices. Our study results reveal that the use of acetylated BSA in PCR micro-devices leads to differential inhibition of PCR, compared to non-acetylated BSA. This result is noticed for the first time, and the differential inhibition generally goes un-noticed, as compared to complete PCR inhibition.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Bovine Serum Albumin solution, 20 mg/mL in H2O, low bioburden, protease free, for molecular biology