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Fate of Escherichia coli O157:H7 and bacterial diversity in corn silage contaminated with the pathogen and treated with chemical or microbial additives.

Journal of dairy science (2017-01-04)
I M Ogunade, Y Jiang, D H Kim, A A Pech Cervantes, K G Arriola, D Vyas, Z G Weinberg, K C Jeong, A T Adesogan
ABSTRACT

Inhibiting the growth of Escherichia coli O157:H7 (EC) in feeds may prevent the transmission or cycling of the pathogen on farms. The first objective of this study was to examine if addition of propionic acid or microbial inoculants would inhibit the growth of EC during ensiling, at silo opening, or after aerobic exposure. The second objective was to examine how additives affected the bacterial community composition in corn silage. Corn forage was harvested at approximately 35% dry matter, chopped to a theoretical length of cut of 10 mm, and ensiled after treatment with one of the following: (1) distilled water (control); (2) 1 × 10

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
LB Broth (Lennox), Highly-referenced microbial growth powder medium, low salt, suitable for salt-sensitive E.coli culture.
Millipore
Mac Conkey-Sorbitol ChromoSelect Agar, suitable for microbiology, NutriSelect® Plus

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