• Home
  • Search Results
  • Evaluation of monoclonal antibodies having specificity for human IgG sub-classes: results of an IUIS/WHO collaborative study.

Evaluation of monoclonal antibodies having specificity for human IgG sub-classes: results of an IUIS/WHO collaborative study.

Immunology letters (1985-01-01)
R Jefferis, C B Reimer, F Skvaril, G de Lange, N R Ling, J Lowe, M R Walker, D J Phillips, C H Aloisio, T W Wells
ABSTRACT

Seventy-four monoclonal antibodies (McAb) of putative specificity for human IgG (11), the IgG sub-classes (59) or Gm allotypes (4) have been evaluated for reactivity and specificity in eight laboratories employing different assay techniques or protocols. For the IgG, IgG3, IgG4, G1m(f) and G3m(u) specificities McAb have been produced that can be satisfactorily applied in most methodologies employed and have potential as reference reagents. The IgG1 and particularly IgG2 specificities proved problematical with all McAb evaluated demonstrating apparent assay restriction and whilst performing well in some assays proved to be poor or inactive reagents in others. However, the study identifies McAb individually suited to application within most commonly employed methodologies. Epitope display is the probable variability rather than capricious behaviour by the McAb. IgG1 and IgG2 were the least immunogenic of the sub-class proteins and there is evidence that epitope display is influenced by the physical and chemical procedures used to immobilize or fix antigen - a common requirement in the assay systems studied.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Anti-Secretory Component (IgA) antibody, Mouse monoclonal, clone GA-1, purified from hybridoma cell culture
Sigma-Aldrich
Anti-Human IgG (Fc specific)-Peroxidase antibody, Mouse monoclonal, clone HP-6017, purified from hybridoma cell culture
Sigma-Aldrich
Anti-Human IgG1-Peroxidase antibody, Mouse monoclonal, clone 8c/6-39, purified from hybridoma cell culture
Sigma-Aldrich
Anti-Human IgG1 (Fc specific) antibody, Mouse monoclonal, clone HP-6001, purified from hybridoma cell culture
Sigma-Aldrich
Anti-Human IgG4-Peroxidase antibody, Mouse monoclonal, clone HP-6025, purified from hybridoma cell culture
Sigma-Aldrich
Anti-Human IgG3 antibody, Mouse monoclonal, clone HP-6050, purified from hybridoma cell culture
Sigma-Aldrich
Anti-Human IgG1 antibody, Mouse monoclonal, clone 8C/6-39, purified from hybridoma cell culture
Sigma-Aldrich
Mouse Anti-Human IgG₁, Fc Fragment Specific (HP6069) Biotin Conjugate, liquid, clone HP6069, Calbiochem®
Sigma-Aldrich
Anti-Human IgG3-Peroxidase antibody, Mouse monoclonal, clone HP-6050, purified from hybridoma cell culture
Sigma-Aldrich
Anti-Human IgG2 antibody, Mouse monoclonal, clone HP-6014, purified from hybridoma cell culture

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.