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Histone Deacetylase Is Required for GA-Induced Programmed Cell Death in Maize Aleurone Layers.

Plant physiology (2017-10-04)
Haoli Hou, Xueke Zheng, Hao Zhang, Mengxia Yue, Yan Hu, Hong Zhou, Qing Wang, Chengshen Xie, Pu Wang, Lijia Li
ABSTRACT

Recent discoveries have shown that epigenetic regulation is an integral part of phytohormone-mediated processes. The phytohormone gibberellin (GA) triggers a series of events in cereal aleurone cells that lead to programmed cell death (PCD), but the signaling cascade mediating GA-induced PCD in cereal aleurone layers remains largely unknown. Here, we showed that histone deacetylase (HDAC) activity gradually increased relative to histone acetyltransferase (HAT) activity, leading to a global decrease in histone H3 and H4 acetylation levels during PCD of maize (Zea mays) embryoless aleurone layers after 3 d of treatment with GA. HDAC inhibition prevented GA-induced PCD in embryoless aleurone cells, whereas HAT inhibition resulted in PCD even in the absence of GA. Hydrogen peroxide concentrations increased in GA- or HAT inhibitor-treated aleurone cells due to reduced levels of reactive oxygen species scavengers. Hydrogen peroxide-treated aleurone cells showed no changes in the activity or expression of HATs and HDACs. We show that it is possible to predict whether epigenetic modification enzymes serve as a regulator of the GA-triggered PCD signaling pathway in maize aleurone layers. Taken together, these findings reveal that HDAC activity is required for GA-induced PCD in maize aleurone layers and regulates PCD via the reactive oxygen species-mediated signal transduction pathway.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Anti-acetyl-Histone H3 Antibody, from rabbit
Sigma-Aldrich
C646, ≥98% (HPLC)
Sigma-Aldrich
Anti-acetyl-Histone H3 (Lys9) Antibody, serum, Upstate®
Sigma-Aldrich
Anti-acetyl-Histone H4 (Lys5) Antibody, serum, from rabbit
Sigma-Aldrich
Anti-RLN3 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution