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Urinary excretion of unmetabolized benzene as an indicator of benzene exposure.

Journal of toxicology and environmental health (1993-03-01)
S Ghittori, M L Fiorentino, L Maestri, G Cordioli, M Imbriani
ABSTRACT

Benzene concentrations in urine samples (Cu, ng/L) from 110 workers exposed to benzene in chemical plants and gasoline pumps were determined by injecting urine supernate into a gas chromatograph. The urine was saturated with anhydrous N2SO4 to facilitate the passage of benzene in the air over the urine. The solvent was stripped from the urine surface and concentrated on an adsorbent substrate (Carbotrap tube) by means of a suction pump (flow rate 150 ml/m). Wash-up of the head space was achieved by simultaneous intake of filtered air through charcoal. Benzene was thermically desorbed and injected in a column (thermal tube disorder, Supelco; 370 degrees C thermal flash; borosilicate capillary glass column SPB-1, 60 m length, 0.75 mm ID, 1 microns film thickness; GC Dani 8580-FID). Benzene concentrations in the urine from 40 non-exposed subjects (20 smokers > 20 cigarette/d and 20 nonsmokers) were also determined [median value of 790 ng/L (10.17 nmol/L) and 131 ng/L (1.70 nmol/L), respectively]. The 8-h time-weighted exposure intensity (Cl, micrograms/m3) of individual workers was monitored by means of charcoal tubes. The median value for exposure to benzene was 736 micrograms/m3 (9.42 mumol/m3) [geometric standard deviation (GSD) = 2.99; range 64 micrograms/m3 (0.82 mumol/m3) to 13,387 micrograms/m3) (171.30 mumol/m3)]. The following linear correlation was found between benzene concentrations in urine (Cu, ng/L) and benzene concentrations in the breathing zone (Cl, micrograms/m3): log(Cu) = 0.645 x log(Cl) + 1.399 r = .559, n = 110, p < .0001 With exclusion of workers who smoked from the study, the correlation between air benzene concentration and benzene measured in urine was: log(Cu) = 0.872 x log(Cl) + 0.6 r = .763, n = 63, p < .0001 The study results indicate that the urinary level of benzene is an indicator of occupational exposure to benzene.

MATERIALS
Product Number
Brand
Product Description

Supelco
Carbotrap® 202, stainless steel TD tube, O.D. × L 1/4 in. × 3 1/2 in., preconditioned, pkg of 10 ea
Supelco
Carbotrap® 217, stainless steel TD tube, O.D. × L 1/4 in. × 3 1/2 in., preconditioned, pkg of 10 ea
Supelco
Carbotrap® 201, for use with Dynatherm ACEM 900/901-FF, I.D. × O.D. × L 1 mm × 6 mm × 7.25 in., pkg of 1 ea
Supelco
Carbotrap® 317, glass TD tube (fast-flow), preconditioned, pkg of 1 ea
Supelco
Carbotrap® 100, glass TD tube, preconditioned, pkg of 10 ea
Supelco
ORBO 100 on Carbotrap® B (20/40), 350/175 mg, W,W,W separators, O.D. × L 7 mm × 110 mm, pkg of 25 ea
Supelco
SPB®-1 Capillary GC Column, L × I.D. 30 m × 0.25 mm, df 0.25 μm
Supelco
ORBO 101 Carbotrap® B (20/40), 100/50 mg, W,W,W separators, O.D. × L 6 mm × 70 mm, pkg of 25 ea
Supelco
Carbotrap® 217, stainless steel TD tube, O.D. × L 1/4 × 3 1/2, unconditioned, pkg of 10 ea
Supelco
Carbotrap® 217, O.D. × L 1/4 in. × 3 1/2 in., stainless steel TD tube, Sealed with Brass Endcaps, preconditioned, pkg of 10 ea
Supelco
Carbotrap® 202, O.D. × L 1/4 in. × 3 1/2 in., stainless steel TD tube, Sealed with Brass Endcaps, preconditioned, pkg of 10 ea
Supelco
Carbotrap® 217, O.D. × L 1/4 in. × 3 1/2 in., glass TD tube, fritted, Sealed with Brass Endcaps, preconditioned, pkg of 10 ea
Supelco
Carbotrap® 217, glass TD tube, fritted, O.D. × L 1/4 in. × 3 1/2 in., preconditioned, pkg of 10 ea
Supelco
Carbotrap® 217, glass TD tube, fritted, O.D. × L 1/4 in. × 3 1/2, unconditioned, pkg of 10 ea
Supelco
Carbotrap® 300, glass TD tube, unconditioned, pkg of 10 ea
Supelco
Carbotrap® 300, O.D. × L 1/4 in. × 3 1/2 in., stainless steel TD tube, preconditioned, pkg of 10 ea
Supelco
Carbotrap® 300, glass TD tube, fritted, O.D. × L 1/4 in. × 3 1/2 in., preconditioned, pkg of 10 ea
Supelco
Carbotrap® 349, glass TD tube, fritted, O.D. × I.D. × L 6 mm × 4 mm × 7 in., preconditioned, pkg of 1 ea
Supelco
Carbotrap® 300, stainless steel TD tube, O.D. × I.D. × L 6 mm × 4 mm × 7 in., preconditioned, pkg of 1 ea
Supelco
Carbotrap® 300, glass TD tube, fritted, O.D. × I.D. × L 6 mm × 4 mm × 4.5 in., preconditioned, pkg of 1 ea
Supelco
Carbotrap® 300, stainless steel TD tube, O.D. × I.D. × L 1/4 in. × 5 mm × 7 in., preconditioned, pkg of 1 ea
Supelco
Carbotrap® Adsorbent, matrix Carbotrap® X, 20-40 mesh, bottle of 10 g
Supelco
Carbotrap® 300, glass TD tube, fritted, O.D. × I.D. × L 6 mm × 4 mm × 7 in., preconditioned, pkg of 1 ea
Supelco
Carbotrap® Adsorbent, matrix Carbotrap® Y, 20-40 mesh, bottle of 10 g
Supelco
Carbotrap® Adsorbent, matrix Carbotrap® C, 20-40 mesh, bottle of 10 g
Supelco
Carbotrap® Adsorbent, matrix Carbotrap® B, 20-40 mesh, bottle of 10 g
Supelco
Carbotrap® Adsorbent, matrix Carbotrap® B, 20-40 mesh, pack of 144 × 190 mg
Supelco
Carbotrap® 200 Dynatherm 890, 850, for use with Dynatherm ACEM-9300, ACEM-900 850/890 TDU, MTDU and OI Analytical Air Tube Desorber or DMP-16
Supelco
Carbotrap® 300, glass TD tube, fritted, O.D. × L 1/4 in. × 3 1/2 in., unconditioned, pkg of 10 ea
Supelco
Carbotrap® 300, stainless steel TD tube, unconditioned, pkg of 10 ea