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[A method for measuring urinary concentrations of benzene. Its use in monitoring of subjects exposed to low levels].

La Medicina del lavoro (1990-03-01)
M L Fiorentino, S Ghittori, G Pezzagno
ABSTRACT

Benzene is a widely diffuse solvent (atmosphere, cigarette smoke, some foods); in the industrial environment benzene is currently present at concentrations of ppm. A valid method of biological monitoring that is easy to perform is needed for assessing occupational and non-occupational exposures. A new method has been developed to evaluate low concentrations of benzene in urine samples by means of a "dynamic" headspace (50 ml of urine in a 120 ml vial). The urine is saturated with anhydrous Na2SO4 in order to support the entrance of benzene in the air over the urine. The solvent is stripped from the urine surface and concentrated on an adsorbent substrate (Carbotrap 100 tube) by means of a suction pump (150 ml/min). A simultaneous intake of filtered air through a charcoal tube allows wash-up of the headspace. Benzene is thermically desorbed and injected in a column (Thermal tube desorber-Supelco; 370 degrees C thermal flash; borosilicate capillary glass column SPB-1 60 m length, 0.75 mm I.D., 1 micron film thickness; G.C. Dani 8580-FID). The detection limit of the method is about 50 ng/l and the variation coefficient is 4.7%. The method was checked on urine samples of 5 non-smokers and 5 smokers: mean values of 135 and 944 ng/l respectively were obtained. A further analysis on urine samples of 60 smokers revealed a significant relationship (p less than 0.001) between urinary benzene concentrations and C0 alveolar concentrations (r = 0.626). A close relationship between benzene exposure levels and urinary concentrations was found in a group of workers exposed to low environmental benzene concentrations (mean value 1200 micrograms/m3) (r = 0.763).

MATERIALS
Product Number
Brand
Product Description

Supelco
Carbotrap® 300, glass TD tube, unconditioned, pkg of 10 ea
Supelco
Carbotrap® 300, O.D. × L 1/4 in. × 3 1/2 in., stainless steel TD tube, preconditioned, pkg of 10 ea
Supelco
Carbotrap® 300, glass TD tube, fritted, O.D. × L 1/4 in. × 3 1/2 in., preconditioned, pkg of 10 ea
Supelco
Carbotrap® 349, glass TD tube, fritted, O.D. × I.D. × L 6 mm × 4 mm × 7 in., preconditioned, pkg of 1 ea
Supelco
Carbotrap® 300, stainless steel TD tube, O.D. × I.D. × L 6 mm × 4 mm × 7 in., preconditioned, pkg of 1 ea
Supelco
Carbotrap® 300, glass TD tube, fritted, O.D. × I.D. × L 6 mm × 4 mm × 4.5 in., preconditioned, pkg of 1 ea
Supelco
Carbotrap® 300, stainless steel TD tube, O.D. × I.D. × L 1/4 in. × 5 mm × 7 in., preconditioned, pkg of 1 ea
Supelco
Carbotrap® Adsorbent, matrix Carbotrap® X, 20-40 mesh, bottle of 10 g
Supelco
Carbotrap® 300, glass TD tube, fritted, O.D. × I.D. × L 6 mm × 4 mm × 7 in., preconditioned, pkg of 1 ea
Supelco
Carbotrap® Adsorbent, matrix Carbotrap® Y, 20-40 mesh, bottle of 10 g
Supelco
Carbotrap® Adsorbent, matrix Carbotrap® C, 20-40 mesh, bottle of 10 g
Supelco
Carbotrap® Adsorbent, matrix Carbotrap® B, 20-40 mesh, bottle of 10 g
Supelco
Carbotrap® Adsorbent, matrix Carbotrap® B, 20-40 mesh, pack of 144 × 190 mg
Supelco
Carbotrap® 200 Dynatherm 890, 850, for use with Dynatherm ACEM-9300, ACEM-900 850/890 TDU, MTDU and OI Analytical Air Tube Desorber or DMP-16
Supelco
Carbotrap® 300, glass TD tube, fritted, O.D. × L 1/4 in. × 3 1/2 in., unconditioned, pkg of 10 ea
Supelco
Carbotrap® 300, stainless steel TD tube, unconditioned, pkg of 10 ea
Supelco
Carbotrap® Adsorbent, matrix Carbotrap® C, 20-40 mesh, bottle of 500 g
Supelco
Carbotrap® 349, O.D. × L 1/4 in. × 3 1/2 in., glass TD tube, fritted, Sealed with Brass Endcaps, preconditioned, pkg of 10 ea
Supelco
Carbotrap® 300, O.D. × L 1/4 in. × 3 1/2 in., stainless steel TD tube, Sealed with Brass Endcaps, preconditioned, pkg of 10 ea
Supelco
Carbotrap® 300, O.D. × L 1/4 in. × 3 1/2 in., glass TD tube, fritted, Sealed with Brass Endcaps, preconditioned, pkg of 10 ea
Supelco
Carbotrap® 202, stainless steel TD tube, O.D. × L 1/4 in. × 3 1/2 in., preconditioned, pkg of 10 ea
Supelco
Carbotrap® 217, stainless steel TD tube, O.D. × L 1/4 in. × 3 1/2 in., preconditioned, pkg of 10 ea
Supelco
Carbotrap® 217, stainless steel TD tube, O.D. × L 1/4 × 3 1/2, unconditioned, pkg of 10 ea
Supelco
Carbotrap® 201, for use with Dynatherm ACEM 900/901-FF, I.D. × O.D. × L 1 mm × 6 mm × 7.25 in., pkg of 1 ea
Supelco
ORBO 101 Carbotrap® B (20/40), 100/50 mg, W,W,W separators, O.D. × L 6 mm × 70 mm, pkg of 25 ea
Supelco
SPB®-1 Capillary GC Column, L × I.D. 30 m × 0.25 mm, df 0.25 μm
Supelco
Carbotrap® 317, glass TD tube (fast-flow), preconditioned, pkg of 1 ea
Supelco
Carbotrap® 100, glass TD tube, preconditioned, pkg of 10 ea
Supelco
ORBO 100 on Carbotrap® B (20/40), 350/175 mg, W,W,W separators, O.D. × L 7 mm × 110 mm, pkg of 25 ea
Supelco
Carbotrap® 217, glass TD tube, fritted, O.D. × L 1/4 in. × 3 1/2, unconditioned, pkg of 10 ea