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  • Comparison of three different solid-phase microextraction fibres for analysis of essential oils in yacon (Smallanthus sonchifolius) leaves.

Comparison of three different solid-phase microextraction fibres for analysis of essential oils in yacon (Smallanthus sonchifolius) leaves.

Journal of chromatography. A (2005-08-24)
M Adam, M Juklová, T Bajer, A Eisner, K Ventura
ABSTRACT

A headspace solid-phase microextraction (HS-SPME) procedure based on three commercialised fibers (100 microm polydimethylsiloxane, 65 microm polydimethylsiloxane-divinylbenzene and 50/30 microm divinylbenzene-Carboxen-polydimethylsiloxane) is presented for the determination of a selected essential oils in dried leaves of yacon (Smallanthus sonchifolius). The extraction performances of these compounds were compared using fibers with one, two and three coatings. The optimal experimental procedures for the adsorption and desorption of target compounds were determined. Significant parameters affecting sorption process such as sample weight, sorption and desorption time and temperature were optimised and discussed. Finally, the optimised procedures were applied successfully for the determination of these compounds in various yacon species. The relative concentration factors of three characteristic components of yacon were measured for relative evaluation of the fiber efficiency. Main essential oils were isolated from dried yacon leaves by appropriate solid-phase microextraction fiber and semi-quantitative analysis of the target volatiles was conducted by gas chromatography-flame ionisation detection (GC-FID) using a capillary column. Three compounds--beta-pinene, caryophylene and y-cadinene were found as the predominant essential oils. Its relative content was important for specification of yacon varieties. Solid-phase microextraction in combination with gas chromatography enabled a rapid and simple determination of relative content of essential oils in yacon.

MATERIALS
Product Number
Brand
Product Description

Supelco
Tenax® TA / Carboxen® 1018, glass TD tube, preconditioned, O.D. × L 1/4  in. × 3 1/2  in., Sealed with (Swagelok® End-Fittings), pkg of 10 ea
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Carboxen®-1010 PLOT Capillary GC Column, L × I.D. 30 m × 0.53 mm, average thickness 30 μm
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Carboxen® Adsorbent, matrix Carboxen® 569, 20-45 mesh, bottle of 10 g
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ORBO 78 HBr on Carboxen® 564 specially cleaned (20/45), 400/200 mg, W,W,W separators, O.D. × L 6 mm × 110 mm, pkg of 25 ea
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Carboxen®-1006 PLOT Capillary GC Column, L × I.D. 30 m × 0.53 mm, average thickness 30 μm
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Carboxen® Adsorbent, matrix Carboxen® 1000, 60-80 mesh, bottle of 10 g
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ORBO 90 Carboxen® 564 (20/45), 160/80 mg, W,F,F separators, O.D. × L 6 mm × 75 mm, pkg of 25 ea, for analyte group MEK (methylethyl ketone)
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Carboxen®-1010 PLOT Capillary GC Column, L × I.D. 30 m × 0.32 mm, average thickness 15 μm
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Carboxen® Adsorbent, matrix Carboxen® 569, 20-45 mesh, bottle of 500 g
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Carboxen®-1006 PLOT Capillary GC Column, L × I.D. 30 m × 0.32 mm, average thickness 15 μm
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Carboxen® Adsorbent, matrix Carboxen® 1003, 40-60 mesh, bottle of 10 g
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Carboxen® Adsorbent, matrix Carboxen® 1000, 40-60 mesh, bottle of 50 g
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Carboxen® Adsorbent, matrix Carboxen® 563, 20-45 mesh, bottle of 10 g
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Carboxen® Adsorbent, matrix Carboxen® 564, 20-45 mesh, bottle of 10 g
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Carboxen® 1000 Reversible SPE Tube, 200 mg/mL, pk 30
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Carboxen® 572 for Tobacco Smoke, Adsorbent Bed Wt: (300 mg), cartridge volume 3 mL, pkg of 10 ea
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ORBO-93 Sorbent Tube, matrix Carboxen® 1000, ORBO tube I.D. × L 6 mm × 95 mm, Bed B 90 mg, Bed A 180 mg, 60/80 mesh, pkg of 25 ea
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SPME fiber assembly Carboxen/Polydimethylsiloxane (CAR/PDMS), df 75 μm (CAR/PDMS), for use with manual holder, needle size 24 ga
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SPME fiber assembly Divinylbenzene/Carboxen/Polydimethylsiloxane (DVB/CAR/PDMS), needle size 24 ga, for use with manual holder
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Carboxen® 569, O.D. × L 1/4  in. × 3 1/2 , glass TD tube, fritted, Sealed with Brass Endcaps, preconditioned, pkg of 10 ea
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Carboxen® 572 for Tobacco Smoke, Adsorbent Bed Wt: 300 mg, cartridge size 3 mL, pkg of 50 ea
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SPME fiber assembly Carboxen/Polydimethylsiloxane (CAR/PDMS), df 75 μm (CAR/PDMS), needle size 23 ga, for use with manual holder
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SPME fiber assembly Divinylbenzene/Carboxen/Polydimethylsiloxane (DVB/CAR/PDMS), needle size 24 ga, StableFlex, for use with manual holder/autosampler
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Carboxen® 569, glass TD tube, fritted, O.D. × L 1/4  in. × 3 1/2  in., preconditioned, pkg of 10 ea
Supelco
Carboxen® Adsorbent, matrix Carboxen® 564, 20-45 mesh, pack of 144 × 290 mg
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SPME fiber assembly Carboxen/Polydimethylsiloxane (CAR/PDMS), df 75 μm (CAR/PDMS), needle size 24 ga, for use with autosampler
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SPME fiber assembly Divinylbenzene/Carboxen/Polydimethylsiloxane (DVB/CAR/PDMS), needle size 24 ga, StableFlex, for use with autosampler
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SPME fiber assembly Carboxen/Polydimethylsiloxane (CAR/PDMS), df 75 μm (CAR/PDMS), needle size 23 ga, for use with autosampler
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SPME fiber assembly Divinylbenzene/Carboxen/Polydimethylsiloxane (DVB/CAR/PDMS), needle size 23 ga, StableFlex, for use with manual holder/autosampler, fiber L 2 cm
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SPME fiber assembly Carboxen/Polydimethylsiloxane (CAR/PDMS), df 85 μm (CAR/PDMS), needle size 24 ga, for use with manual holder, StableFlex fiber